DEG/ENaC but not TRP channels are the major mechanoelectrical transduction channels in a C. elegans nociceptor.
DEG/ENaC but not TRP channels are the major mechanoelectrical transduction channels in a C. elegans nociceptor.
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DOI：
10.1016/j.neuron.2011.06.038
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发表时间：
2011-09-08
期刊：
影响因子：
16.2
通讯作者：
Goodman MB
中科院分区：
文献类型：
--
作者：
Geffeney SL;Cueva JG;Glauser DA;Doll JC;Lee TH;Montoya M;Karania S;Garakani AM;Pruitt BL;Goodman MB
许多伤害感受器检测机械信号，但在这些感觉神经元中负责机械传导的离子通道仍然不清楚。使用在体内记录和遗传解剖，我们确定了DEG/ENaC蛋白，DEG-1，作为主要的机械转导通道的ASH，多模态伤害感受器在秀丽隐杆线虫。但是，DEG-1不是ASH中唯一的机械转导通道：DEG-1的丢失揭示了一个较小的电流，其性质与DEG/ENaC通道的预期不同。该电流独立于在ASH中表达的两个TRPV通道。虽然这些TRPV通道的损失抑制对伤害性刺激的行为反应，我们发现，这两个mechanoreceptor电流和电位基本上是野生型的TRPV突变体。我们建议，ASH伤害感受器依赖于两个遗传上不同的机械转导通道和TRPV通道有助于编码和传递信息。因为哺乳动物和昆虫的伤害感受器也共表达DEG/ENaC和TRPV，所以这里阐述的这些离子通道的细胞功能可能是保守的。
Many nociceptors detect mechanical cues, but the ion channels responsible for mechanotransduction in these sensory neurons remain obscure. Using in vivo recordings and genetic dissection, we identified the DEG/ENaC protein, DEG-1, as the major mechanotransduction channel in ASH, a polymodal nociceptor in Caenorhabditis elegans. But, DEG-1 is not the only mechanotransduction channel in ASH: loss of deg-1 revealed a minor current whose properties differ from those expected of DEG/ENaC channels. This current was independent of two TRPV channels expressed in ASH. Although loss of these TRPV channels inhibits behavioral responses to noxious stimuli, we found that both mechanoreceptor currents and potentials were essentially wild-type in TRPV mutants. We propose that ASH nociceptors rely on two genetically-distinct mechanotransduction channels and that TRPV channels contribute to encoding and transmitting information. Because mammalian and insect nociceptors also co-express DEG/ENaCs and TRPVs, the cellular functions elaborated here for these ion channels may be conserved.
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