Interferon-beta treatment normalises the inhibitory effect of serum from multiple sclerosis patients on oligodendrocyte progenitor proliferation
Interferon-beta treatment normalises the inhibitory effect of serum from multiple sclerosis patients on oligodendrocyte progenitor proliferation
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干扰素-β 治疗使多发性硬化症患者血清对少突胶质祖细胞增殖的抑制作用正常化
DOI：
10.1016/j.neulet.2010.08.075
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发表时间：
2010
影响因子：
2.5
通讯作者：
M. Stangel
中科院分区：
文献类型：
--
作者：
A. Kotsiari;E. Voss;R. Pul;T. Skripuletz;Daniela Ragancokova;C. Trebst;M. Stangel
干扰素-β（IFN-β）是复发缓解型多发性硬化症（MS）的既定疗法。然而，其作用方式和对少突胶质细胞的影响尚不清楚。在这项研究中，我们研究了IFN-β治疗对混合胶质细胞培养物中原代少突胶质前体细胞（OPC）增殖和分化的影响。将混合的神经胶质培养物在补充有来自健康对照、未治疗的MS患者和IFN-β治疗的MS患者的10%血清的培养基中孵育5天。免疫细胞化学法检测OPC的增殖和分化。与健康对照相比，未治疗的MS患者的血清显著抑制OPC的增殖，而IFN-β治疗的MS患者的血清几乎完全逆转了这种作用。未观察到对OPC分化的影响。对第二组接受IFN-β治疗的MS患者进行的前瞻性和纵向分析显示，OPC增殖抑制的逆转在治疗12个月后明显，但在前6个月内不明显。因此，我们的结果表明，IFN-β治疗有能力恢复MS患者血清对OPC增殖的抑制作用。目前尚不清楚这对再生过程意味着什么。
Interferon-beta (IFN-β) is an established therapy for relapsing-remitting multiple sclerosis (MS). However, the mode of action and the effect on oligodendrocytes are not yet clear. In this study, we examined the influence of an IFN-β therapy on the proliferation and differentiation of primary oligodendrocyte precursor cells (OPC) in mixed glial cultures. Mixed glial cultures were incubated for 5 days in medium supplemented with 10% of sera from healthy controls, untreated MS patients and IFN-β treated MS patients. Proliferation and differentiation of OPC were determined by immunocytochemistry. Proliferation of OPC was significantly inhibited by sera from untreated MS patients compared to healthy controls, while this effect was almost completely reversed by serum from IFN-β treated MS patients. No effect on OPC differentiation was observed. A prospective and longitudinal analysis of a second cohort of MS patients treated with IFN-β showed that the reversal of inhibition of OPC proliferation was evident after 12 months of treatment but not during the first 6 months. Thus, our results suggest that IFN-β treatment has the capacity to revert the inhibitory effect of serum from MS patients on OPC proliferation. It is currently not clear what this means for regenerative processes.
影响因子：
14.5
作者：
Patrikios, Peter;Stadelmann, Christine;Lassmann, Hans
通讯作者：
Lassmann, Hans