Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers.
Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers.
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使用带有荧光 RNA 适体的修饰 sgRNA 的 CRISPR-dCas13 系统进行活细胞 RNA 成像
DOI：
10.1039/d2sc04656c
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发表时间：
2022-12-07
期刊：
影响因子：
8.4
通讯作者：
中科院分区：
文献类型：
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作者：
The development of RNA imaging strategies in live cells is essential to improve our understanding of their role in various cellular functions. We report an efficient RNA imaging method based on the CRISPR-dPspCas13b system with fluorescent RNA aptamers in sgRNA (CasFAS) in live cells. Using modified sgRNA attached to fluorescent RNA aptamers that showed reduced background fluorescence, this approach provides a simple, sensitive way to image and track endogenous RNA with high accuracy and efficiency. In addition, color switching can be easily achieved by changing the fluorogenic dye analogues in living cells through user-friendly washing and restaining operations. CasFAS is compatible with orthogonal fluorescent aptamers, such as Broccoli and Pepper, enabling multiple colors RNA labeling or intracellular RNA–RNA interaction imaging. Finally, the visualization of severe fever with thrombocytopenia syndrome virus (SFTSV) was achieved by CasFAS, which may facilitate further studies on this virus.
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DOI：
10.1126/science.1202142
发表时间：
2011-04-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Larson DR;Zenklusen D;Wu B;Chao JA;Singer RH
通讯作者：
Singer RH
影响因子：
48
作者：
Ma H;Tu LC;Naseri A;Chung YC;Grunwald D;Zhang S;Pederson T
通讯作者：
Pederson T
DOI：
10.1126/science.1207339
发表时间：
2011-07-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Paige JS;Wu KY;Jaffrey SR
通讯作者：
Jaffrey SR
DOI：
10.1083/jcb.201604115
发表时间：
2016-08-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ma H;Tu LC;Naseri A;Huisman M;Zhang S;Grunwald D;Pederson T
通讯作者：
Pederson T
RIC-seq 用于 RNA–RNA 空间相互作用的全局原位分析
影响因子：
64.8
作者：
Cai, Zhaokui;Cao, Changchang;Xue, Yuanchao
通讯作者：
Xue, Yuanchao