Immune-Mediated Subepithelial Blistering Diseases of the Mucous Membranes: Improving the Detection of Circulating Autoantibodies by the Use of Concentrated Serum Samples
Immune-Mediated Subepithelial Blistering Diseases of the Mucous Membranes: Improving the Detection of Circulating Autoantibodies by the Use of Concentrated Serum Samples
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免疫介导的粘膜上皮下起泡疾病：通过使用浓缩血清样本改进循环自身抗体的检测
DOI：
10.1001/archderm.1996.03890340054009
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发表时间：
1996
影响因子：
--
通讯作者：
R. D. Watson
中科院分区：
文献类型：
--
作者：
N. Korman;R. D. Watson
背景与设计：对疑似免疫介导性粘膜上皮下水泡性疾病患者浓缩前后血清样本循环自身抗体检测进行比较。我们确定使用疑似免疫介导的粘膜上皮下水泡性疾病患者的浓缩血清样本是否提高了循环抗体的诊断敏感性。我们研究了13名来自大学转诊诊所的患者，他们没有皮肤损伤，也没有粘膜上皮下疤痕形成的水泡病。其中3名患者有可检测到的循环自身抗体，10名患者使用标准技术进行的间接免疫荧光研究结果为阴性。血清样本浓缩后的主要观察指标为间接免疫荧光法、免疫印迹法和免疫沉淀法检测盐裂皮肤循环自身抗体。结果：10例常规方法未检出循环自身抗体的患者中，5例(50%)检出循环免疫球蛋白抗体，3例(30%)检出循环免疫球蛋白A抗体。在用标准技术检测到循环自身抗体的3例患者中，1例(33%)循环IgA抗体仅在使用浓缩血清样本时才免疫印迹97kd线性IgA大疱病抗原。结论：使用浓缩血清样本可以提高我们在可疑但无法分类的免疫介导性黏膜上皮下水泡病患者中检测循环自身抗体的能力和抗原特异性。阿奇皮马托尔。1996年；132：1194-1198
Background and Design: Comparison of detection of circulating autoantibodies before and after concentration of serum samples from patients with suspected immunemediated subepithelial blistering diseases of the mucous membranes. We determine whether the use of concentrated serum samples from patients with suspected immune-mediated subepithelial blistering diseases of the mucous membranes improves diagnostic sensitivity for circulating antibodies. We studied 13 patients from a university-based referral practice who had no skin lesions and a scarring subepithelial blistering disease of the mucous membranes. Three of these patients had detectable circulating autoantibodies and 10 had negative indirect immunofluorescence study results using standard techniques. The main outcome measures after concentration of serum samples were detection of circulating autoantibodies on salt-split skin by indirect immunofluorescence, immunoblotting, and immunoprecipitation. Results: Of the 10 patients in whom circulating autoantibodies had not been detectable with standard techniques, circulating IgG antibodies were detected in 5 (50%) and circulating IgA antibodies in 3 (30%). Of the 3 patients in whom circulating autoantibodies had been detectable with standard techniques, 1 (33%) had circulating IgA antibodies that immunoblotted the 97-kd linear IgA bullous disease antigen only when concentrated serum samples were used. Conclusions: The use of concentrated serum samples can improve our ability to detect the presence and antigenic specificity of circulating autoantibodies in patients with suspected but unclassifiable immunemediated subepithelial blistering diseases of the mucous membranes. Arch Dermatol. 1996;132:1194-1198
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DOI：
10.1016/0030-4220(93)90140-y
发表时间：
1993
期刊：
Oral surgery, oral medicine, and oral pathology
影响因子：
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作者：
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通讯作者：
Anhalt,GJ
DOI：
10.1111/1523-1747.ep12275033
发表时间：
1985
期刊：
The Journal of investigative dermatology
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作者：
Meyer,JR;Migliorati,CA;Daniels,TE;Greenspan,JS
通讯作者：
Greenspan,JS
影响因子：
6.5
作者：
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影响因子：
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作者：
Domloge-Hultsch,N;Bisalbutra,P;Gammon,WR;Yancey,KB
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Yancey,KB
用于对瘢痕性类天疱疮患者血清进行免疫荧光筛查的盐裂人体皮肤基质以及 IgA 抗体免疫沉淀的新方法。
影响因子：
13.8
作者：
Sarret,Y;Hall,R;Cobo,LM;Thivolet,J;Patton,DL;Woodley,DT
通讯作者：
Woodley,DT