Cross-reactive serum and memory B-cell responses to spike protein in SARS-CoV-2 and endemic coronavirus infection.
Cross-reactive serum and memory B-cell responses to spike protein in SARS-CoV-2 and endemic coronavirus infection.
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DOI：
10.1038/s41467-021-23074-3
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发表时间：
2021-05-19
影响因子：
16.6
通讯作者：
Andrabi R
中科院分区：
文献类型：
--
作者：
Song G;He WT;Callaghan S;Anzanello F;Huang D;Ricketts J;Torres JL;Beutler N;Peng L;Vargas S;Cassell J;Parren M;Yang L;Ignacio C;Smith DM;Voss JE;Nemazee D;Ward AB;Rogers T;Burton DR;Andrabi R
对季节性地方性冠状病毒的预先存在的免疫可能对自然感染或疫苗接种诱导的SARS-CoV-2抗体应答产生深远影响。确定预先存在的反应是否会影响SARS-CoV-2感染的第一步是了解人类对冠状病毒交叉反应的性质和程度。在这里，我们比较血清抗体和记忆B细胞的反应，冠状病毒刺突蛋白从大流行前和SARS-CoV-2恢复期捐助者使用结合和功能测定。我们发现在大流行前的献血者中存在SARS-CoV-2交叉反应血清抗体的证据很弱。然而，我们发现了在SARS-CoV-2感染期间激活的预先存在的交叉反应记忆B细胞的证据。单克隆抗体与β冠状病毒（包括SARS-CoV-1和地方性冠状病毒）显示出不同程度的交叉反应性。我们鉴定出一种针对S蛋白S2亚基的交叉反应中和抗体。我们的研究结果表明，在评估SARS-CoV-2抗体应答时，应考虑对地方性冠状病毒的预先存在的免疫力。抗原相关病毒之间预先存在的免疫应答可以影响病毒感染或疫苗接种中的应答。在这里，作者评估和表征的抗体和记忆B细胞群体特异性的SARS-CoV 2和地方性人类冠状病毒的存在。
Pre-existing immunity to seasonal endemic coronaviruses could have profound consequences for antibody responses to SARS-CoV-2, induced from natural infection or vaccination. A first step to establish whether pre-existing responses can impact SARS-CoV-2 infection is to understand the nature and extent of cross-reactivity in humans to coronaviruses. Here we compare serum antibody and memory B cell responses to coronavirus spike proteins from pre-pandemic and SARS-CoV-2 convalescent donors using binding and functional assays. We show weak evidence of pre-existing SARS-CoV-2 cross-reactive serum antibodies in pre-pandemic donors. However, we find evidence of pre-existing cross-reactive memory B cells that are activated during SARS-CoV-2 infection. Monoclonal antibodies show varying degrees of cross-reactivity with betacoronaviruses, including SARS-CoV-1 and endemic coronaviruses. We identify one cross-reactive neutralizing antibody specific to the S2 subunit of the S protein. Our results suggest that pre-existing immunity to endemic coronaviruses should be considered in evaluating antibody responses to SARS-CoV-2. Pre-existing immune responses between antigenically related viruses can influence responses in viral infections or vaccinations. Here the authors assess and characterize the presence of antibody and memory B cell populations specific to SARS-CoV2 and endemic human coronaviruses.
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影响因子：
16.6
作者：
Cao L;Pauthner M;Andrabi R;Rantalainen K;Berndsen Z;Diedrich JK;Menis S;Sok D;Bastidas R;Park SR;Delahunty CM;He L;Guenaga J;Wyatt RT;Schief WR;Ward AB;Yates JR 3rd;Burton DR;Paulson JC
通讯作者：
Paulson JC
影响因子：
64.5
作者：
Briney, Bryan;Sok, Devin;Jardine, Joseph G.;Kulp, Daniel W.;Skog, Patrick;Menis, Sergey;Jacak, Ronald;Kalyuzhniy, Oleksandr;de Val, Natalia;Sesterhenn, Fabian;Le, Khoa M.;Ramos, Alejandra;Jones, Meaghan;Saye-Francisco, Karen L.;Blane, Tanya R.;Spencer, Skye;Georgeson, Erik;Hu, Xiaozhen;Ozorowski, Gabriel;Adachi, Yumiko;Kubitz, Michael;Sarkar, Anita;Wilson, Ian A.;Ward, Andrew B.;Nemazee, David;Burton, Dennis R.;Schief, William R.
通讯作者：
Schief, William R.
影响因子：
64.5
作者：
Bonsignori M;Zhou T;Sheng Z;Chen L;Gao F;Joyce MG;Ozorowski G;Chuang GY;Schramm CA;Wiehe K;Alam SM;Bradley T;Gladden MA;Hwang KK;Iyengar S;Kumar A;Lu X;Luo K;Mangiapani MC;Parks RJ;Song H;Acharya P;Bailer RT;Cao A;Druz A;Georgiev IS;Kwon YD;Louder MK;Zhang B;Zheng A;Hill BJ;Kong R;Soto C;NISC Comparative Sequencing Program;Mullikin JC;Douek DC;Montefiori DC;Moody MA;Shaw GM;Hahn BH;Kelsoe G;Hraber PT;Korber BT;Boyd SD;Fire AZ;Kepler TB;Shapiro L;Ward AB;Mascola JR;Liao HX;Kwong PD;Haynes BF
通讯作者：
Haynes BF
影响因子：
3.7
作者：
Elshabrawy HA;Coughlin MM;Baker SC;Prabhakar BS
通讯作者：
Prabhakar BS
影响因子：
64.8
作者：
Arvin, Ann M.;Fink, Katja;Virgin, Herbert W.
通讯作者：
Virgin, Herbert W.