Translational Initiation Factor eIF-4E
Translational Initiation Factor eIF-4E
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翻译起始因子 eIF-4E
DOI：
--
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发表时间：
1996
影响因子：
4.8
通讯作者：
P. McDermott
中科院分区：
文献类型：
--
作者：
H. Wada;Charles T. Ivester;B. Carabello;G. Cooper;P. McDermott
为了确定心脏负荷和蛋白质合成速率之间的耦合机制，在施加负荷后测量eIF-4 E磷酸化程度的变化。电刺激收缩的成年猫心肌细胞增加eIF-4 E磷酸化到34%，4小时后，与8%的磷酸化在静止的控制。然而，eIF-4 E磷酸化没有增加电刺激后，在存在7.5 mM的2，3-丁二酮单肟，肌动蛋白-肌球蛋白跨桥循环和主动张力发展的抑制剂。用0.1 μM胰岛素或0.1 μM佛波醇12-肉豆蔻酸酯13-乙酸酯处理成年心肌细胞，eIF-4 E磷酸化分别增加到2,3%和64%，但这些增加不被2，3-丁二酮单肟阻断。在犬体内急性血流动力学超负荷模型中，左心室压力超负荷时eIF-4 E磷酸化增加至23%，而对照组为7%。急性容量超负荷对eIF-4 E磷酸化没有影响。eIF-4 E磷酸化的这些变化解释了对急性压力与急性容量超负荷的合成代谢反应的差异。这些数据表明，eIF-4 E磷酸化是一种机制，通过这种机制，心脏负荷增加与蛋白质合成速率加快相结合。
To define the coupling mechanism between cardiac load and the rate of protein synthesis, changes in the extent of eIF-4E phosphorylation were measured after imposition of a load. Electrically stimulated contraction of adult feline cardiocytes increased eIF-4E phosphorylation to 34% after 4 h, as compared with 8% phosphorylation in quiescent controls. However, eIF-4E phosphorylation did not increase upon electrical stimulation in the presence of 7.5 mM 2,3-butanedione monoxime, an inhibitor of actin-myosin cross-bridge cycling and active tension development. Treatment of adult cardiocytes with either 0.1 μM insulin or 0.1 μM phorbol 12-myristate 13-acetate increased eIF-4E phosphorylation to 23 and 64%, respectively, but these increases were not blocked by 2,3-butanedione monoxime. In canine models of acute hemodynamic overload in vivo, eIF-4E phosphorylation increased to 23% in response to left ventricular pressure overload as compared with 7% phosphorylation in controls. Acute volume overload had no effect on eIF-4E phosphorylation. These changes in eIF-4E phosphorylation account for differences in anabolic responses to acute pressure versus acute volume overload. These data suggest that eIF-4E phosphorylation is a mechanism by which increased cardiac load is coupled to accelerated rates of protein synthesis.
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kimball,SR;Jefferson,LS
通讯作者：
Jefferson,LS
影响因子：
18.2
作者：
H. E. Morgan;E. E. Gordon-E.;Y. Kira;B H L Chua;L A Russo;C. J. Peterson;P J McDermott;P A Watson
通讯作者：
H. E. Morgan;E. E. Gordon-E.;Y. Kira;B H L Chua;L A Russo;C. J. Peterson;P J McDermott;P A Watson