Comparison of the diets of the parrotfishes Scarus ovifrons and Calotomus japonicus using rDNA metabarcoding

Comparison of the diets of the parrotfishes Scarus ovifrons and Calotomus japonicus using rDNA metabarcoding
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使用 rDNA 元条形码比较鹦嘴鱼 Scarus ovifrons 和 Calotomus japonicus 的饮食

DOI:
10.1007/s12562-022-01623-z
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发表时间:
2022
期刊:
影响因子:
1.9
通讯作者:
Haruo Yamaguchi & Masao Adachi
Haruo Yamaguchi & Masao Adachi
中科院分区:
农林科学4区
文献类型:
--
作者:
Chiho Homma;Daiki Inokuchi;Yohei Nakamura;Kouhei Ohnishi;Hiroshi Funaki;Haruo Yamaguchi & Masao Adachi

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鹦鹉,卵鳞鱼和日本石斑鱼,生活在日本暖温带的珊瑚礁上。在这两个人中,只有一个。卵裂会导致严重的食物中毒,而导致食物中毒的毒素被认为是通过食物链积累的。然而,这两种鹦鹉在猎物有机体上的差异还没有被很好地理解。因此,在本研究中,我们对肠道内容物进行了代谢编码分析(真核生物的18S rDNA和蓝藻的16S rDNA)。卵叶和C.日本想要澄清这些食物来源。两种鹦鹉肠道内容物中的大型微生物(> 1 mm)主要由红藻门、白藻门和线虫纲组成,微生物(< 1 mm)主要由甲藻、节肢动物和绿藻组成。此外,在两种鱼中都检测到蓝藻。内脏内容物OFS。卵叶中含有较高比例的红藻序列,而OFC中含有较高比例的红藻序列。日本鱼含有较高比例的白藻门序列,只有巨藻、甲藻和蓝藻等生物序列在日本鱼中检测到。卵子。我们的研究阐明了不同性别的人在肠道内容物组成上的异同。卵叶和C.为阐明BYS中毒的致病微生物提供了基础信息。卵子。
The parrotfishesScarus ovifronsandCalotomus japonicusinhabit the warm temperate reefs of Japan. Of the two, onlyS. ovifronscauses severe food poisoning, and the toxin that causes the food poisoning is believed to accumulate through the food chain. However, the difference in prey organisms between the two parrotfishes is not well understood. Therefore, in this study, we conducted metabarcoding analysis (18S rDNA for eukaryotes and 16S rDNA for cyanobacteria) of the gut contents ofS. ovifronsandC. japonicusto clarify those food resources. Macroorganisms (> 1 mm) in the gut contents of the two parrotfishes were mainly composed of Rhodophyta, Ochrophyta, and Cnidaria sequences, and microorganisms (< 1 mm) were mainly composed of Dinoflagellata, Arthropoda, and Chlorophyta. In addition, cyanobacteria were detected in both fishes. The gut contents ofS. ovifronscontained a high proportion of Rhodophyta sequences, whereas those ofC. japonicuscontained a high proportion of Ochrophyta sequences, and some organism sequences such as Gigartinales (Rhodophyta), dinoflagellates, and cyanobacteria were detected in onlyS. ovifrons. Our study clarified similarities and differences in the gut content composition betweenS. ovifronsandC. japonicus, providing fundamental information for elucidating the causative organism of poisoning byS. ovifrons.
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发表时间: 2010-04-01
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发表时间: 2020
期刊: Harmful algae
影响因子: 6.6
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