The Immunomodulatory Functions of Various CpG Oligodeoxynucleotideson CEF Cells and H(9)N(2) Subtype Avian Influenza Virus Vaccination.
The Immunomodulatory Functions of Various CpG Oligodeoxynucleotideson CEF Cells and H(9)N(2) Subtype Avian Influenza Virus Vaccination.
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DOI：
10.3390/vaccines10040616
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发表时间：
2022-04-14
期刊：
影响因子：
7.8
通讯作者：
Feng, Xiuli
中科院分区：
文献类型：
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作者：
Li, Chenfei;Huang, Xiangyu;Cai, Jiaxi;Lu, Anran;Hao, Shanshan;Zhang, Ze;Sun, Haifeng;Feng, Xiuli
CpG寡脱氧核苷酸（CpG ODN）对抗原蛋白具有佐剂活性，可诱导机体产生针对抗原的体液和细胞免疫应答。然而，不同序列的CpG ODN的免疫调节功能有很大差异。本文以CpG 2007为模板，设计了6个不同序列的CpG ODN。通过对CEF细胞的体外筛选，检测CpG ODNs对CEF细胞的刺激活性。然后，通过用硫取代氧（S-CpG）来修饰所选择的两个CpG ODN序列骨架并验证免疫活性。接下来，为了证明S-CpG作为免疫增强剂的可行性，在20日龄的鸡疫苗接种中制备了具有或不具有白色油佐剂的两种免疫模型。体外筛选实验表明，CpG ODN 4和5的诱导作用能强烈刺激多种免疫相关分子的表达。此外，经硫酸化修饰的CpG ODN 4和5可显著诱导多种细胞因子的表达。CpG ODN 4和5在免疫过程中诱导了较强的体液免疫和细胞免疫应答，其中白色油作为佐剂能显著提高CpG ODN的免疫效果。这些结果为探讨CpG ODN的结构特征和疫苗免疫提供了重要的实验依据。
CpG oligodeoxynucleotides (CpG ODN) present adjuvant activities for antigen proteins, which can induce humoral and cellular immune responses to antigens. However, the immunomodulatory functions of CpG ODNs with different sequences are very different. In this paper, six CpG ODNs with different sequences were designed based on CpG2007 as a template. Through the screening of CEF cells in vitro, the stimulating activity of CpG ODNs was determined. Then, two selected CpG ODN sequence backbones were modified by substituting the oxygen with sulfur (S-CpG) and verifying the immune activity. Next, to prove the feasibility of S-CpG as an immune potentiator, two immune models with or without white oil adjuvant were prepared in 20-day-old chicken vaccinations. The screening experiment in vitro showed that the inducing roles of CpG ODN 4 and 5 could strongly stimulate various immune-related molecular expressions. Additionally, CpG ODN 4 and 5 with sulfation modification significantly induced various cytokines’ expressions. Furthermore, CpG ODN 4 and 5 induced the strongly humoral and cellular immune responses during vaccination, in which white oil, as an adjuvant, could significantly improve the immune effect of CpG ODN. These results provide an important experimental basis for exploring the structural characteristics and vaccine immunity of CpG ODN.
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影响因子：
7.8
作者：
Chuang YC;Tseng JC;Yang JX;Liu YL;Yeh DW;Lai CY;Yu GY;Hsu LC;Huang CM;Chuang TH
通讯作者：
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影响因子：
7.8
作者：
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影响因子：
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通讯作者：
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影响因子：
8
作者：
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通讯作者：
Hanagata N
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影响因子：
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作者：
Butterfield, John S. S.;Biswas, Moanaro;Herzog, Roland W.
通讯作者：
Herzog, Roland W.