Basophil Activation Test: Old and New Applications in Allergy.
Basophil Activation Test: Old and New Applications in Allergy.
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DOI：
10.1007/s11882-018-0831-5
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发表时间：
2018-11-15
影响因子：
5.5
通讯作者：
Santos AF
中科院分区：
文献类型：
--
作者：
Hemmings O;Kwok M;McKendry R;Santos AF
嗜碱性粒细胞激活试验（BAT）取代了传统的组胺等介质释放法，可用于临床，也可用于研究效应细胞对变应原的免疫应答机制。本文综述了近年来BAT在变态反应的临床、诊断和实验室研究中的进展。作为在患病患者体内发生的过敏反应的体外替代物，BAT可用于支持各种过敏状况的诊断，例如食物、药物、呼吸道和昆虫毒液过敏，以及评估对过敏原特异性免疫疗法和其他免疫调节治疗的临床反应。BAT也可用于研究目的，以探索嗜碱性粒细胞水平的过敏和耐受机制，例如通过操纵IgE和IgG及其受体，以及通过研究响应过敏原的细胞内信号级联。本文综述了BAT在过敏性疾病临床治疗中的应用，以及近年来对过敏原免疫应答机制的认识和技术进步。
The basophil activation test (BAT) using flow cytometry has supplanted traditional methods of measuring basophil degranulation using histamine and other mediator release, and can be used for clinical applications as well as to explore the immune mechanisms of effector cell response to allergen. This review discusses the advancements made in clinical, diagnostic and laboratory research of allergy utilizing an ever-evolving BAT. Being an in vitro surrogate of the allergic reaction that happens in vivo in the sick patient, the BAT can be used to support the diagnosis of various allergic conditions, such as food, drug, respiratory and insect venom allergies, and the assessment of clinical response to allergen-specific immunotherapy and other immunomodulatory treatments. The BAT can also be used for research purposes to explore the mechanisms of allergy and tolerance at the level of the basophil, for instance by manipulating IgE and IgG and their receptors and by studying intracellular signalling cascade in response to allergen. This review covers the applications of the BAT to the clinical management of allergic patients and the increased understanding of the mechanisms of immune response to allergens as well as technological advancements made in recent years.
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