Simultaneous determination of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β -D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography.
Simultaneous determination of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β -D-glucuronopyranside methyl ester and quercetin in effective part of Polygonum Perfoliatum L. using high performace liquid chromatography.
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DOI：
10.4103/0973-1296.137379
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发表时间：
2014-07
影响因子：
0.7
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Fan D;Zhao Y;Zhou X;Gong X;Zhao C
建立了高效液相色谱-二极管阵列检测器（HPLC-PD）法同时测定扛叶蓼中槲皮素、槲皮素-3-O-β-D-葡萄糖醛酸苷、槲皮素-3-O-β-D-吡喃葡萄糖醛酸苷甲酯和槲皮素的含量。采用反相C18色谱柱，以乙腈-0.5%醋酸水溶液为流动相，采用梯度洗脱，移动的流动相为流动相。分析物的校准曲线在研究范围内具有良好的线性。在方法验证程序中，所开发分析方法的日内和日间精密度、重复性和稳定性均符合要求。所建立的方法的回收率范围为95.76%至102.10%的所有分析物。该方法已成功地应用于杠板归有效部位中4种化合物的同时测定。来自不同的地区。采用系统聚类分析和主成分分析方法对样品进行了定性和定量分析。建立的HPLC法结合化学计量学方法可有效控制托叶蓼药材的质量。
In the present study, a high performance liquid chromatography (HPLC) coupled with photodiode array detection was developed for simultaneous quantitation of esculetin, quercetin-3-O-β-D-glucuronide, quercetin-3-O-β-D- glucuronopyranoside methyl ester and quercetin in Polygonum perfoliatum L. The chromatographic separations were performed on a reversed-phase C18 column using a mobile phase composed of acetonitrile -0.5% aqueous acetic acid with gradient elution. The calibration curves for the analytes demonstrated good linearities within the investigated ranges. The satisfactory intra- and inter-day precision, repeatability and stability of the developed analytical method were shown in the method validation procedure. The recoveries of the established method ranged from 95.76 to 102.10% for all the analytes. This proposed method was successfully applied for simultaneous quantification of the four compounds in effective part of Polygonum perfoliatum L. from different regions. Hierarchical clustering analysis (HCA) and principal components analysis (PCA) were performed to characterize and classify the samples based on the contents of the four compounds in Polygonum perfoliatum L. The established HPLC method combined with chemometric approaches was proven to be useful and efficient for quality control of Polygonum perfoliatum L.
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DOI：
10.3724/sp.j.1145.2009.00615
发表时间：
2009-01-01
影响因子：
--
作者：
Li Hongfang;Ma Qingyun;Zhao Youxing
通讯作者：
Zhao Youxing
影响因子：
1.7
作者：
Shen, T.;Jia, Z.-J.;Zheng, S.-Z.
通讯作者：
Zheng, S.-Z.
影响因子：
6.1
作者：
Lin, WL;Wang, CJ;Tseng, TH
通讯作者：
Tseng, TH
影响因子：
6.1
作者：
Shirai, M;Moon, JH;Terao, J
通讯作者：
Terao, J
影响因子：
5.6
作者：
Prabakaran, Dhamodaran;Ashokkumar, Natarajan
通讯作者：
Ashokkumar, Natarajan