A new genetic locus for self-compatibility in the outcrossing grass species perennial ryegrass (Lolium perenne).
A new genetic locus for self-compatibility in the outcrossing grass species perennial ryegrass (Lolium perenne).
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DOI：
10.1093/aob/mcaa140
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发表时间：
2021-05-07
期刊：
影响因子：
4.2
通讯作者：
Thorogood D
中科院分区：
文献类型：
--
作者：
Slatter LM;Barth S;Manzanares C;Velmurugan J;Place I;Thorogood D
自交不亲和性（SI）是许多开花植物用来防止自花受精和维持杂合性的生理机制。在禾本科植物中，已知这是由两个基因座（S-Z）系统控制的；然而，SI 系统本质上是有漏洞的。已知 S 和 Z 基因座以及另一个基因座 T 的修饰基因会覆盖 SI，从而导致自体受精和自体种子产生。这对生态和进化的成功以及草的商业育种具有影响。在这里，我们报告了一项研究，其中自交亲和性（SC）的遗传控制是通过对来自单种子血统产生的两个独立衍生的自交系的多年生黑麦草 F2 群体进行自花授粉的结果来确定的。 对 73 株可育植物的体外自花授粉进行了分析。使用一组 1863 个单核苷酸多态性 (SNP) 标记进行遗传关联分析，这些标记是通过基因型测序方法生成的。标记被放置在使用 Joinmap v.5 创建的七个连锁群 (LG) 的重组图上。在 143 株植物上测定了自花授粉和开放授粉花序上的种子，其中包括分析自花授粉反应的 73 株植物。自花授粉显示 SC 百分比呈双峰分布，峰值分别为 50% 和 100%。鉴定出单个数量性状基因座 (QTL) 与映射到 LG 6 的标记 6S14665z17875_11873 的峰值关联。峰值位置与最大标记分离扭曲相关。自交亲和植物在自花授粉和开放授粉后同样繁殖。这是禾本科中首次报道位于 LG 6 上的 SC 基因座。这一新的 SC QTL 发现，以及表明花粉-柱头识别过程的复杂性及其进化意义，为培育多年生黑麦草提供了额外的 SC 来源。
Self-incompatibility (SI) is a physiological mechanism that many flowering plants employ to prevent self-fertilization and maintain heterozygosity. In the grass family this is known to be controlled by a two locus (S-Z) system; however, the SI system is intrinsically leaky. Modifier genes of both the S and Z loci and a further locus, T, are known to override SI leading to self-fertilization and self-seed production. This has implications for the ecological and evolutionary success as well as the commercial breeding of grasses. Here we report a study where the genetic control of self-compatibility (SC) was determined from the results of self-pollinating an F2 population of perennial ryegrass from two independently derived inbred lines produced by single-seed descent. In vitro self-pollinations of 73 fertile plants were analysed. A genetic association analysis was made with a panel of 1863 single-nucleotide polymorphism (SNP) markers, generated through genotype-by-sequencing methodology. Markers were placed on a recombination map of seven linkage groups (LGs) created using Joinmap v.5. The seed set on self- and open-pollinated inflorescences was determined on 143 plants, including the 73 plants analysed for self-pollination response. Self-pollinations revealed a bimodal distribution of percentage SC with peaks at 50 and 100 %. A single quantitative trait locus (QTL) was identified with peak association for marker 6S14665z17875_11873 that mapped to LG 6. Peak position was associated with maximum marker segregation distortion. The self-compatible plants were equally fecund after self- and open pollination. This is the first report in the Poaceae family of an SC locus located on LG 6. This new SC QTL discovery, as well as indicating the complex nature of the pollen–stigma recognition process and its evolutionary significance, provides an additional source of SC for breeding perennial ryegrass.
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影响因子：
3.8
作者：
HALEY, CS;KNOTT, SA
通讯作者：
KNOTT, SA
影响因子：
3.8
作者：
CORNISH, MA;HAYWARD, MD;LAWRENCE, MJ
通讯作者：
LAWRENCE, MJ
影响因子：
3.8
作者：
HAYMAN, DL;RICHTER, J
通讯作者：
RICHTER, J
影响因子：
5.4
作者：
MARTINEZ, O;CURNOW, RN
通讯作者：
CURNOW, RN
影响因子：
0.4
作者：
Fernandes, E. H.;Schuster, I.;Coan, M. M. D.
通讯作者：
Coan, M. M. D.