Piceatannol induces regulatory T cells and modulates the inflammatory response and adipogenesis.
Piceatannol induces regulatory T cells and modulates the inflammatory response and adipogenesis.
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DOI：
10.1016/j.biopha.2023.114514
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发表时间：
2023-05
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影响因子：
--
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中科院分区：
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--
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多酚化合物云杉烷醇（PC）的有益效果已被报道用于代谢疾病、抗增殖、抗氧化和抗癌特性。尽管它对炎症性疾病有有益的作用，但对PC如何调节炎症反应和脂肪生成知之甚少。因此，本研究旨在确定PC对炎症反应和脂肪生成的影响。通过流式细胞术、qRT-PCR、形态测定和蛋白质印迹分析来分析PC对脾细胞、3 T3-L1脂肪细胞和RAW264.7巨噬细胞的影响。与对照组相比，PC以剂量依赖性方式使用刺激的脾细胞诱导活化的T细胞凋亡，并减少T细胞的活化，改变T细胞频率，并有趣地诱导调节性T（Treg）细胞的频率。与对照组相比，PC可抑制LPS诱导的RAW264.7巨噬细胞TNF-α、iNOS、IL-6 R的表达和NF-κB的活化。有趣的是，PC改变了3 T3-L1脂肪细胞的细胞形态，伴随着细胞体积、脂质沉积和TNF-α表达的减少，但瘦素和IL-1β的上调。我们的研究结果表明，PC诱导活化的T细胞凋亡，降低免疫细胞活化和炎症反应，并阻碍脂肪形成。这组新的数据为治疗炎症性疾病和肥胖症的新疗法提供了有希望的希望。
The beneficial effects of the polyphenolic compound piceatannol (PC) has been reported for metabolic diseases, antiproliferative, antioxidant, and anti-cancer properties. Despite its beneficial effects on inflammatory diseases, little is known about how PC regulates inflammatory responses and adipogenesis. Therefore, this study was designed to determine the effects of PC on the inflammatory response and adipogenesis. The effect of PC on splenocytes, 3T3-L1 adipocytes, and RAW264.7 macrophages was analyzed by flow cytometry, qRT-PCR, morphometry, and western blot analysis. PC induced apoptosis in activated T cells in a dose-dependent manner using stimulated splenocytes and reduced the activation of T cells, altered T cell frequency, and interestingly induced the frequency of regulatory T (Treg) cells as compared to controls. PC suppressed the expression of TNF-α, iNOS, IL-6R, and NF-κB activation in RAW264.7 macrophages after lipopolysaccharides (LPS)-induction as compared to the control. Interestingly, PC altered the cell morphology of 3T3-L1 adipocytes with a concomitant decrease in cell volume, lipid deposition, and TNF-α expression, but upregulation of leptin and IL-1β. Our findings suggested that PC induced apoptosis in activated T cells, decreased immune cell activation and inflammatory response, and hindered adipogenesis. This new set of data provides promising hope as a new therapeutic to treat both inflammatory disease and obesity.
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