Serum analysis of tryptophan catabolism pathway: correlation with Crohn's disease activity.
Serum analysis of tryptophan catabolism pathway: correlation with Crohn's disease activity.
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DOI：
10.1002/ibd.21849
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发表时间：
2012-07
影响因子：
4.9
通讯作者：
Ciorba, Matthew A.
中科院分区：
文献类型：
--
作者：
Gupta, Nitin K.;Thaker, Ameet I.;Kanuri, Navya;Riehl, Terrence E.;Rowley, Christopher W.;Stenson, William F.;Ciorba, Matthew A.
吲哚胺2，3双加氧酶1（Indoleamine 2，3 dioxygenase 1，IDO 1）是一种色氨酸分解酶，具有免疫耐受促进作用。我们试图确定克罗恩病（CD）中IDO 1的肠道表达增加是否会导致色氨酸和初始IDO 1途径分解代谢物犬尿氨酸的血清水平的可检测变化。个体通过华盛顿大学消化疾病研究中心前瞻性入组。蒙特利尔分类用于疾病表型。根据医生的总体评估对疾病严重程度进行分类。用高压液相色谱法测定血清色氨酸和犬尿氨酸。在福尔马林固定的组织块上进行IDO 1免疫组织化学染色。入选25例CD患者和11例对照。8例CD患者在两个不同的时间点和疾病活动水平收集血清。与对照组相比，在活动性CD期间，固有层和上皮中均存在强IDO 1表达。抑制血清色氨酸水平和犬尿氨酸/色氨酸（K/T）的比例升高，发现与缓解或对照人群相比，在个人与活跃的CD。K/T比值与疾病活动度、C-反应蛋白和红细胞沉降率呈正相关。在两次血清测量的CD患者亚组中，色氨酸水平升高，而犬尿氨酸水平和K/T比值随着疾病活动的减轻而降低。克罗恩病患者IDO 1表达与血清色氨酸水平降低和K/T比值升高相关这些水平可作为肠粘膜免疫活化的合理客观标志物和克罗恩病活性的替代物。
Indoleamine 2,3 dioxygenase 1 (IDO1) is a tryptophan catabolizing enzyme with immunotolerance promoting functions. We sought to determine if increased gut expression of IDO1 in Crohn’s disease (CD) would result in detectable changes in serum levels of tryptophan and the initial IDO1 pathway catabolite, kynurenine. Individuals were prospectively enrolled through the Washington University Digestive Diseases Research Center. Montreal classification was used for disease phenotyping. Disease severity was categorized by physician’s global assessment. Serum tryptophan and kynurenine were measured by high pressure liquid chromatography. IDO1 immunohistochemical staining was performed on formalin-fixed tissue blocks. 25 CD patients and 11 controls were enrolled. 8 CD patients had serum collected at two different time points and levels of disease activity. Strong IDO1 expression exists in both the lamina propria and epithelium during active CD compared to controls. Suppressed serum tryptophan levels and an elevated kynurenine/tryptophan (K/T) ratio were found in individuals with active CD as compared to those in remission or the control population. K/T ratios correlated positively with disease activity as well as with C-reactive protein and erythrocyte sedimentation rate. In the subgroup of CD patients with two serum measurements, tryptophan levels elevated while kynurenine levels and the K/T ratio lowered as the disease activity lessened. IDO1 expression in Crohn’s disease is associated with lower serum tryptophan and an elevated K/T ratio. These levels may serve a reasonable objective marker of gut mucosal immune activation and surrogate for Crohn’s Disease activity.
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影响因子：
29.4
作者：
Gurtner, GJ;Newberry, RD;Stenson, WF
通讯作者：
Stenson, WF
影响因子：
3.1
作者：
Huttunen, Reetta;Syrjanen, Jaana;Hurme, Mikko
通讯作者：
Hurme, Mikko
影响因子：
10.5
作者：
Booij, L;Van Der Does, AJW;McNally, RJ
通讯作者：
McNally, RJ
影响因子：
11
作者：
通讯作者：
--
DOI：
10.4049/jimmunol.0900291
发表时间：
2010-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ciorba MA;Bettonville EE;McDonald KG;Metz R;Prendergast GC;Newberry RD;Stenson WF
通讯作者：
Stenson WF