cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors.
cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors.
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cAMP 和 c-di-GMP 通过其效应器的直接相互作用协同支持生物膜维护
DOI：
10.1038/s41467-022-29240-5
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发表时间：
2022-03-21
影响因子：
16.6
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Liu C;Sun D;Liu J;Chen Y;Zhou X;Ru Y;Zhu J;Liu W
核苷酸第二信使，如cAMP和c-di-GMP，调节细菌的许多生理过程，包括生物膜的形成。有证据表明，c-di-GMP介导的通路和cAMP受体蛋白(CRP)介导的通路之间存在串扰，但机制往往不清楚。在这里，我们表明，cAMP-CRP不仅通过其对基因转录的已知影响，而且通过与内膜上可能的c-di-GMP效应物BpfD的直接相互作用来调节腐败希万氏菌的生物膜维持。CAMP-CRP与BpfD的结合增强了BpfD与蛋白酶BpfG的已知相互作用，从而阻止了细胞表面相关粘附素BPFA的蛋白降解处理和释放，从而有助于生物膜的维护。我们的结果提供了cAMP和c-di-GMP信号通路之间通过直接相互作用的证据，并表明cAMP-CRP可以在翻译后水平上发挥调节作用。核苷酸第二信使，如cAMP和c-di-GMP，调节细菌的许多生理过程，包括生物膜的形成。在这里，作者提供了cAMP和c-di-GMP通路之间通过其效应器直接相互作用的证据，表明cAMP受体蛋白(CRP)可以在翻译后水平上发挥调节作用。
Nucleotide second messengers, such as cAMP and c-di-GMP, regulate many physiological processes in bacteria, including biofilm formation. There is evidence of cross-talk between pathways mediated by c-di-GMP and those mediated by the cAMP receptor protein (CRP), but the mechanisms are often unclear. Here, we show that cAMP-CRP modulates biofilm maintenance in Shewanella putrefaciens not only via its known effects on gene transcription, but also through direct interaction with a putative c-di-GMP effector on the inner membrane, BpfD. Binding of cAMP-CRP to BpfD enhances the known interaction of BpfD with protease BpfG, which prevents proteolytic processing and release of a cell surface-associated adhesin, BpfA, thus contributing to biofilm maintenance. Our results provide evidence of cross-talk between cAMP and c-di-GMP pathways through direct interaction of their effectors, and indicate that cAMP-CRP can play regulatory roles at the post-translational level. Nucleotide second messengers, such as cAMP and c-di-GMP, regulate many physiological processes in bacteria, including biofilm formation. Here, the authors provide evidence of cross-talk between cAMP and c-di-GMP pathways through direct interaction of their effectors, showing that the cAMP receptor protein (CRP) can play regulatory roles at the post-translational level.
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影响因子：
10.5
作者：
Dahlstrom KM;O'Toole GA
通讯作者：
O'Toole GA
FlrA 抑制腐败希瓦氏菌中生物膜相关 bpfA 操纵子的转录
影响因子：
4.4
作者：
Cheng, Yuan-Yuan;Wu, Chao;Xiao, Ya-Zhong
通讯作者：
Xiao, Ya-Zhong
影响因子：
6.4
作者：
Giacalone D;Smith TJ;Collins AJ;Sondermann H;Koziol LJ;O'Toole GA
通讯作者：
O'Toole GA
影响因子：
5.6
作者：
Belyaeva, TA;Rhodius, VA;Busby, SJW
通讯作者：
Busby, SJW
影响因子：
2.8
作者：
Almblad, Henrik;Rybtke, Morten;Tolker-Nielsen, Tim
通讯作者：
Tolker-Nielsen, Tim