Molecular characterization of a chromosomal locus in Staphylococcus aureus that contributes to oxidative defence and is highly induced by the cell-wall-active antibiotic oxacillin.
Molecular characterization of a chromosomal locus in Staphylococcus aureus that contributes to oxidative defence and is highly induced by the cell-wall-active antibiotic oxacillin.
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金黄色葡萄球菌染色体位点的分子特征，该位点有助于氧化防御，并受到细胞壁活性抗生素苯唑西林的高度诱导。
DOI：
10.1099/00221287-147-11-3037
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Jayaswal,RK
中科院分区：
文献类型：
--
作者：
Singh,VK;Moskovitz,J;Wilkinson,BJ;Jayaswal,RK
以前的研究采用二维凝胶电泳和N-末端蛋白质测序表明，甲硫氨酸亚砜还原酶（MsrA）的合成升高在金黄色葡萄球菌响应细胞壁活性抗生素。在本研究中，S.金黄色葡萄球菌msrA基因被克隆、过表达、纯化为His标记的MsrA，并显示具有甲硫氨酸亚砜还原酶活性。通过测定msrA启动子：：lacZ融合株中β-半乳糖苷酶活性和北方印迹分析研究msrA的转录。转录ofmsrA增加苯唑西林，但不是由各种其他应力，包括H2 O2。北方印迹分析表明，该转录本大小为2·3 kb，明显大于531 ntmsrAORF。该基因转录起始位点位于srA起始密码子上游25 nt处。从数据库序列的计算机分析表明，至少有三个额外的ORF下游的msrA。这三个ORF中的两个的推导的氨基酸序列显示出显着的序列同源性PilB，和酶IIA的磷酸转移酶系统，分别。第三个ORF不能通过同源性搜索鉴定。北方杂交结果表明，S.金黄色葡萄球菌msrA被转录为多顺反子信息的一部分。有趣的是，purifiedS. aureusPilB显示具有比MsrA高28倍的甲硫氨酸亚砜还原酶活性。该操纵子第一个基因的插入敲除突变导致突变株对H2 O2的敏感性增加，但对苯唑西林的敏感性没有增加。
Previous studies employing two-dimensional gel electrophoresis and N-terminal protein sequencing have shown elevated synthesis of the enzyme methionine sulfoxide reductase (MsrA) inStaphylococcus aureusin response to cell-wall-active antibiotics. In the present study, theS. aureus msrAgene was cloned, overexpressed, purified as His-tagged MsrA and shown to have methionine sulfoxide reductase activity. The transcription ofmsrAwas studied by assaying β-galactosidase activity in anmsrApromoter::lacZfusion strain and by Northern blot analysis. Transcription ofmsrAwas increased by oxacillin; but not by a variety of other stresses including H2O2. Northern blot analysis revealed that the size of themsrAtranscript was 2·3 kb, considerably larger than the 531 ntmsrAORF. ThemsrAtranscription start site was mapped 25 nt upstream of themsrAstart codon. Computer analysis from database sequences indicated at least three additional ORFs downstream ofmsrA. The deduced amino acid sequences of two of these three ORFs showed significant sequence homologies to PilB, and enzyme IIA of the phosphotransferase system, respectively. The third ORF could not be identified by homology searches. Northern blot hybridization with probes specific to themsrAdownstream region indicated that theS. aureus msrAwas transcribed as part of a polycistronic message. Interestingly, purifiedS. aureusPilB was shown to possess ∼∼28-fold higher methionine sulfoxide reductase activity than the MsrA. An insertional knockout mutation in the first gene of this operon resulted in increased susceptibility of the mutant to H2O2compared to the parent strain, but not to oxacillin.
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抗氧化防御中的甲硫氨酸亚砜还原酶。
影响因子：
--
作者：
Moskovitz,J;Berlett,BS;Poston,JM;Stadtman,ER
通讯作者：
Stadtman,ER
淋病奈瑟菌中的菌毛蛋白表达受到正转录和负转录控制。
DOI：
10.1002/j.1460-2075.1988.tb03335.x
发表时间：
1988
期刊：
The EMBO journal
影响因子：
--
作者：
Taha,MK;So,M;Seifert,HS;Billyard,E;Marchal,C
通讯作者：
Marchal,C
DOI：
10.1111/j.1432-1033.1992.tb16740.x
发表时间：
1992-03-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
AUGUSTIN, J;ROSENSTEIN, R;GOTZ, F
通讯作者：
GOTZ, F
用于鉴定体内表达基因的链球菌报告基因融合载体。
DOI：
10.1006/plas.1999.1408
发表时间：
1999
期刊：
Plasmid.
影响因子：
--
作者：
Kili,AO;Herzberg,MC;Meyer,MW;Zhao,X;Tao,L
通讯作者：
Tao,L
影响因子：
2.9
作者：
Lowther, WT;Brot, N;Matthews, BW
通讯作者：
Matthews, BW