Tenogenic differentiation of human MSCs induced by the topography of electrochemically aligned collagen threads.
Tenogenic differentiation of human MSCs induced by the topography of electrochemically aligned collagen threads.
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DOI：
10.1016/j.biomaterials.2011.11.066
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发表时间：
2012-03
期刊：
影响因子：
14
通讯作者：
Akkus, Ozan
中科院分区：
文献类型：
--
作者：
Kishore, Vipuil;Bullock, Whitney;Sun, Xuanhao;Van Dyke, William Scott;Akkus, Ozan
来自细胞外微环境的地形线索可以影响细胞活动，包括增殖和分化。关于材料形貌对人间充质干细胞（人 MSC）肌腱分化影响的信息有限。我们的实验室之前开发了一种利用等电聚焦原理的方法来合成电化学排列胶原蛋白 (ELAC) 线，该线模仿胶原蛋白致密结缔组织的堆积密度、排列和强度。在目前的研究中，人间充质干细胞在ELAC和随机定向的胶原线上培养，并研究了胶原定向对细胞形态、增殖和肌腱分化的影响。结果表明，与 ELAC 线相比，随机定向的胶原线的增殖率更高。另一方面，与随机定向的胶原线相比，ELAC 线上的肌腱特异性标记物（例如巩膜、肌腱调节蛋白、肌腱蛋白-C 和胶原蛋白-III）显着增加。此外，骨钙素（一种骨分化的特定标记物）在 ELAC 线上受到抑制。先前的研究报道BMP-12是诱导人MSCs肌腱分化的关键生长因子。为了评估 BMP-12 和胶原蛋白定向的协同效应，在补充和不补充 BMP-12 的培养基中的 ELAC 线上培养人 MSC。结果显示，BMP-12 对 ELAC 线上的人 MSC 的肌腱分化没有额外的影响。总之，这些结果表明 ELAC 通过提供类似于肌腱本身的排列且致密的胶原基质来诱导人类 MSC 的肌腱分化。总之，ELAC 具有用作肌腱替代物和开发骨腱结构以实现骨-肌腱界面再生的巨大潜力。
Topographical cues from the extracellular microenvironment can influence cellular activity including proliferation and differentiation. Information on the effects of material topography on tenogenic differentiation of human mesenchymal stem cells (human MSCs) is limited. A methodology using the principles of isoelectric focusing has previously been developed in our laboratory to synthesize electrochemically aligned collagen (ELAC) threads that mimics the packing density, alignment and strength of collagen dense connective tissues. In the current study, human MSCs were cultured on ELAC and randomly-oriented collagen threads and the effect of collagen orientation on cell morphology, proliferation and tenogenic differentiation was investigated. The results indicate that higher rates of proliferation were observed on randomly oriented collagen threads compared to ELAC threads. On the other hand, tendon specific markers such as scleraxis, tenomodulin, tenascin-C and collagen-III were significantly increased on ELAC threads compared to randomly oriented collagen threads. Additionally, osteocalcin, a specific marker of bone differentiation was suppressed on ELAC threads. Previous studies have reported that BMP-12 is a key growth factor to induce tenogenic differentiation of human MSCs. To evaluate the synergistic effect of BMP-12 and collagen orientation, human MSCs were cultured on ELAC threads in culture medium supplemented with and without BMP-12. The results revealed that BMP-12 did not have an additional effect on the tenogenic differentiation of human MSCs on ELAC threads. Together, these results suggest that ELAC induces tenogenic differentiation of human MSCs by presenting an aligned and dense collagen substrate, akin to the tendon itself. In conclusion, ELAC has a significant potential to be used as a tendon replacement and in the development of an osteotendinous construct towards the regeneration of bone-tendon interfaces.
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影响因子：
1.1
作者：
KRACKOW, KA;THOMAS, SC;JONES, LC
通讯作者：
JONES, LC
影响因子：
4.1
作者：
Kuo, Catherine K.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.
DOI：
10.1002/jbm.b.31962
发表时间：
2012-02-01
影响因子：
3.4
作者：
Kishore, Vipuil;Uquillas, Jorge Alfredo;Akkus, Ozan
通讯作者：
Akkus, Ozan
影响因子：
2.8
作者：
Awad, HA;Boivin, GP;Butler, DL
通讯作者：
Butler, DL
影响因子：
5.3
作者：
Docheva, D;Hunziker, EB;Brandau, O
通讯作者：
Brandau, O