Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch.
Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch.
复制标题
DOI：
10.1016/j.chembiol.2008.09.004
复制
发表时间：
2008-10-20
影响因子：
--
通讯作者：
Ferré-D'Amaré AR
中科院分区：
文献类型：
--
作者：
Xiao H;Edwards TE;Ferré-D'Amaré AR
四环素适体是一种体外选择的RNA，它与抗生素结合的小分子亲和力（Kd ~ 0.8 nM）是已知的人工RNA中最高的。它是已知能够调节体内基因表达的少数适体之一。适配体的2.2 Å-resolution共晶结构揭示了由11个核苷酸环和不规则螺旋的小凹槽之间的三级相互作用形成的假结状褶皱。四环素以镁离子螯合物的形式结合在这个界面内。该结构结合之前的生化和生物物理数据表明，适体在与四环素结合的同时经历了局部折叠。这种人工RNA的三螺旋结，h形结构比大多数适体更复杂，让人想起一些天然的核开关。
The tetracycline aptamer is an in vitro selected RNA that binds to the antibiotic with the highest known affinity of an artificial RNA for a small molecule (Kd ~ 0.8 nM). It is one of few aptamers known to be capable of modulating gene expression in vivo. The 2.2 Å-resolution cocrystal structure of the aptamer reveals a pseudoknot-like fold formed by tertiary interactions between a 11-nucleotide loop and the minor groove of an irregular helix. Tetracycline binds within this interface as a magnesium ion chelate. The structure, together with previous biochemical and biophysical data, indicates that the aptamer undergoes localized folding concomitant with tetracycline binding. The three-helix junction, h-shaped architecture of this artificial RNA is more complex than those of most aptamers and is reminiscent of those of some natural riboswitches.
登录
查看更多内容
影响因子：
64.5
作者：
Brodersen, DE;Clemons, WM;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
影响因子：
64.8
作者：
Batey, RT;Gilbert, SD;Montange, RK
通讯作者：
Montange, RK
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL
影响因子：
5.6
作者：
Ferré-D'Amaré, AR;Doudna, JA
通讯作者：
Doudna, JA
影响因子：
5.7
作者：
Edwards, Thomas E.;Ferre-D'Amare, Adrian R.
通讯作者：
Ferre-D'Amare, Adrian R.