Differential expression of several molecules of the extracellular matrix in functionally and developmentally distinct regions of rat spinal cord
Differential expression of several molecules of the extracellular matrix in functionally and developmentally distinct regions of rat spinal cord
复制标题
大鼠脊髓功能和发育不同区域中细胞外基质几种分子的差异表达
DOI：
10.1007/s00441-006-0289-y
复制
发表时间：
2007
影响因子：
3.6
通讯作者：
S. Biasi
中科院分区：
文献类型：
--
作者：
L. Vitellaro‐Zuccarello;P. Bosisio;S. Mazzetti;C. Monti;S. Biasi
我们研究了几种硫酸软骨素蛋白聚糖（神经蛋白聚糖、brevican蛋白聚糖、versican蛋白聚糖、aggrecan蛋白聚糖、phosphacan蛋白聚糖）及其糖胺聚糖部分和tenascin-R在成年大鼠脊髓中的区域分布。这些分子与神经胶质和神经元群体的关系，用适当的标记物鉴定，用多重荧光标记结合共聚焦显微镜研究。结果表明，所检测的分子在所有脊髓水平上的分布是相似的，但沿背-腹侧轴显示出区域特异性差异，划定了功能和发育不同的区域。在灰质中，I和II层缺乏细胞外基质的神经元周围网（PNNs），并且含有低水平的硫酸软骨素糖胺聚糖（CS-GAGs）、brevican和tenascin-R，这可能有利于局部神经可塑性的维持。相反，在III-VIII和x层中，CS-GAGs， brevican和phosphacan是丰富的，具有许多厚的PNNs，运动神经元（IX层）被PNNs包围，这些PNNs包含所有研究的分子，但显示出不同数量的CS-GAGs。双标记实验表明，pnn的存在不能明确地与特定类别的神经元相关，如运动神经元或通过钙结合蛋白（小白蛋白、钙结合蛋白、钙钙结合蛋白）表达而鉴定的中间神经元。然而，富含CS-GAGs的PNNs与钾通道Kv3.1b亚基（快速放电神经元的标志）的神经元表达之间存在良好的相关性。这一观察证实了这些神经元的电生理特性与其微环境的特定组成之间的相关性。
We have examined the regional distribution of several chondroitin sulfate proteoglycans (neurocan, brevican, versican, aggrecan, phosphacan), of their glycosaminoglycan moieties, and of tenascin-R in the spinal cord of adult rat. The relationships of these molecules with glial and neuronal populations, identified with appropriate markers, were investigated by using multiple fluorescence labeling combined with confocal microscopy. The results showed that the distribution of the examined molecules was similar at all spinal cord levels but displayed area-specific differences along the dorso-ventral axis, delimiting functionally and developmentally distinct areas. In the gray matter, laminae I and II lacked perineuronal nets (PNNs) of extracellular matrix and contained low levels of chondroitin sulfate glycosaminoglycans (CS-GAGs), brevican, and tenascin-R, possibly favoring the maintenance of local neuroplastic properties. Conversely, CS-GAGs, brevican, and phosphacan were abundant, with numerous thick PNNs, in laminae III-VIII and X. Motor neurons (lamina IX) were surrounded by PNNs that contained all molecules investigated but displayed various amounts of CS-GAGs. Double-labeling experiments showed that the presence of PNNs could not be unequivocally related to specific classes of neurons, such as motor neurons or interneurons identified by their expression of calcium-binding proteins (parvalbumin, calbindin, calretinin). However, a good correlation was found between PNNs rich in CS-GAGs and the neuronal expression of the Kv3.1b subunit of the potassium channel, a marker of fast-firing neurons. This observation confirms the correlation between the electrophysiological properties of these neurons and the specific composition of their microenvironment.
登录
查看更多内容
DOI：
10.1073/pnas.94.19.10116
发表时间：
1997-09-16
影响因子：
11.1
作者：
Aspberg, A;Miura, R;Yamaguchi, Y
通讯作者：
Yamaguchi, Y
影响因子：
1.7
作者：
Carr, PA;Alvarez, FJ;Fyffe, REW
通讯作者：
Fyffe, REW
针对结缔组织蛋白聚糖的单克隆抗体的生产和表征。
DOI：
--
发表时间：
1985
期刊：
Federation proceedings
影响因子：
--
作者：
Caterson,B;Christner,JE;Baker,JR;Couchman,JR
通讯作者：
Couchman,JR
DOI：
10.1016/s0021-9258(18)98755-7
发表时间：
1991-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
U. Rauch;P. Gao;A. Janetzko;A. Flaccus;Lutz G. W. Hilgenberg;H. Tekotte;R. Margolis;R. U. Margolis
通讯作者：
U. Rauch;P. Gao;A. Janetzko;A. Flaccus;Lutz G. W. Hilgenberg;H. Tekotte;R. Margolis;R. U. Margolis