Beneficial effects of running exercise on hippocampal microglia and neuroinflammation in chronic unpredictable stress-induced depression model rats.
Beneficial effects of running exercise on hippocampal microglia and neuroinflammation in chronic unpredictable stress-induced depression model rats.
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跑步运动对慢性不可预测应激抑郁模型大鼠海马小胶质细胞和神经炎症的有益影响
DOI：
10.1038/s41398-021-01571-9
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发表时间：
2021-09-06
影响因子：
6.8
通讯作者：
Tang Y
中科院分区：
文献类型：
--
作者：
Xiao K;Luo Y;Liang X;Tang J;Wang J;Xiao Q;Qi Y;Li Y;Zhu P;Yang H;Xie Y;Wu H;Tang Y
跑步运动已被证明可以缓解抑郁症状，但其抗抑郁作用的机制尚不清楚。小胶质细胞和伴随的失调的神经炎症在抑郁症的发病机制中起着关键作用。然而，跑步运动对抑郁症大鼠海马神经炎症及小胶质细胞数量和激活的影响尚未见报道。在本研究中，大鼠接受了5周的慢性不可预测应激(CUS)，然后进行了6周的跑台运动。用蔗糖偏好试验(SPT)评估大鼠的抑郁样症状。用免疫组织化学和体视学方法测定小胶质细胞总数，免疫荧光法测定海马亚区Iba1+/CD68+细胞密度。用定量逆转录聚合酶链式反应和酶联免疫吸附试验检测大鼠海马区促炎细胞因子的水平。结果表明，跑步运动逆转了CUS诱导的抑郁大鼠降低的蔗糖偏好。此外，CUS增加了大鼠海马区小胶质细胞的数量和小胶质细胞的激活，但跑步运动减弱了CUS诱导的海马区小胶质细胞数量的增加和海马齿状回小胶质细胞的激活。此外，CUS显著增加了海马炎性因子的水平，而跑步运动抑制了海马炎性因子的增加。这些结果表明，运动的抗抑郁作用可能是通过减少小胶质细胞的数量，抑制小胶质细胞的激活和海马神经炎症来实现的。
Running exercise has been shown to relieve symptoms of depression, but the mechanisms underlying the antidepressant effects are unclear. Microglia and concomitant dysregulated neuroinflammation play a pivotal role in the pathogenesis of depression. However, the effects of running exercise on hippocampal neuroinflammation and the number and activation of microglia in depression have not been studied. In this study, rats were subjected to chronic unpredictable stress (CUS) for 5 weeks followed by treadmill running for 6 weeks. The depressive-like symptoms of the rats were assessed with a sucrose preference test (SPT). Immunohistochemistry and stereology were performed to quantify the total number of ionized calcium-binding adapter molecule 1 (Iba1)+microglia, and immunofluorescence was used to quantify the density of Iba1+/cluster of differentiation 68 (CD68)+in subregions of the hippocampus. The levels of proinflammatory cytokines in the hippocampus were measured by qRT-PCR and ELISA. The results showed that running exercise reversed the decreased sucrose preference of rats with CUS-induced depression. In addition, CUS increased the number of hippocampal microglia and microglial activation in rats, but running exercise attenuated the CUS-induced increases in the number of microglia in the hippocampus and microglial activation in the dentate gyrus (DG) of the hippocampus. Furthermore, CUS significantly increased the hippocampal levels of inflammatory factors, and the increases in inflammatory factors in the hippocampus were suppressed by running exercise. These results suggest that the antidepressant effects of exercise may be mediated by reducing the number of microglia and inhibiting microglial activation and neuroinflammation in the hippocampus.
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慢性不可预测应激引起的抑郁小鼠模型中小胶质细胞刺激的抗抑郁特性
DOI：
10.1016/j.pnpbp.2020.109931
发表时间：
2020-07-13
影响因子：
5.6
作者：
Cai, Zixuan;Ye, Ting;Huang, Chao
通讯作者：
Huang, Chao
影响因子：
34.7
作者：
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通讯作者：
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影响因子：
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通讯作者：
Fukuuchi, Y
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影响因子：
5.8
作者：
He, Ming-chao;Shi, Zhe;Zhang, Yan
通讯作者：
Zhang, Yan
影响因子：
5.1
作者：
Ignacio, Zuleide M.;da Silva, Renato S.;Reus, Gislaine Z.
通讯作者：
Reus, Gislaine Z.