The responses to long-term nitrogen addition of soil bacterial, fungal, and archaeal communities in a desert ecosystem.
The responses to long-term nitrogen addition of soil bacterial, fungal, and archaeal communities in a desert ecosystem.
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DOI：
10.3389/fmicb.2022.1015588
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发表时间：
2022
影响因子：
5.2
通讯作者：
Tan, Dunyan
中科院分区：
文献类型：
--
作者：
Zhang, Xuan;Song, Xin;Wang, Taotao;Huang, Lei;Ma, Haiyang;Wang, Mao;Tan, Dunyan
氮（N）沉降是人类活动引起的世界性问题。氮的长期沉降强烈影响植物生产力和群落组成。然而，目前尚不清楚微生物群落如何响应沙漠生态系统中的长期氮添加。为此，2015年在中国西北古尔班通古特沙漠进行了长期试验，试验了0（CK）、5（N1）、20（N2）和80（N3）kg N ha−1 yr.−1四种施氮量，并在施氮6年后对土壤进行了采样。高通量测序（HTS）用于分析土壤微生物组成。 HTS结果表明，氮的添加对细菌的α多样性和β多样性没有显着影响（p > 0.05），但显着降低了古菌的β多样性（p < 0.05）。 N2处理的真菌Chao1和ACE指数分别增加了24.10%和26.07%。此外，氮的添加影响细菌和真菌的群落结构。例如，与CK相比，N3处理下放线菌门相对丰度增加了17.80%，拟杆菌门相对丰度降低了44.46%。此外，氮的添加还改变了细菌和真菌群落的功能。 N3处理显示硝酸盐还原菌的相对丰度增加（比CK高27.06%）。 N1处理中共生真菌的相对丰度增加（比CK高253.11%）。 SOC和NH4+-N可以解释62%的真菌群落功能变化。 N添加可直接影响细菌群落功能或通过NO3−-N间接影响细菌群落功能。这些结果表明不同的微生物群可能对氮的添加有不同的反应。与细菌和真菌相比，氮添加对古菌群落的影响较小。同时，氮介导的土壤性质变化在微生物群落的变化中发挥着重要作用。本研究的结果为了解沙漠生态系统中的微生物群落如何适应长期氮沉降提供了可靠的基础。
Nitrogen (N) deposition is a worldwide issue caused by human activity. Long-term deposition of N strongly influences plant productivity and community composition. However, it is still unclear how the microbial community responds to long-term N addition in a desert ecosystem. Therefore, a long-term experiment was conducted in the Gurbantonggut Desert in northwestern China in 2015. Four N addition rates, 0 (CK), 5 (N1), 20 (N2), and 80 (N3) kg N ha−1 yr.−1, were tested and the soil was sampled after 6 years of N addition. High-throughput sequencing (HTS) was used to analyze the soil microbial composition. The HTS results showed that N addition had no significant effect on the bacterial α-diversity and β-diversity (p > 0.05) but significantly reduced the archaeal β-diversity (p < 0.05). The fungal Chao1 and ACE indexes in the N2 treatment increased by 24.10 and 26.07%, respectively. In addition, N addition affected the bacterial and fungal community structures. For example, compared to CK, the relative abundance of Actinobacteria increased by 17.80%, and the relative abundance of Bacteroidetes was reduced by 44.46% under N3 treatment. Additionally, N addition also changed the bacterial and fungal community functions. The N3 treatment showed increased relative abundance of nitrate-reducing bacteria (27.06% higher than CK). The relative abundance of symbiotrophic fungi was increased in the N1 treatment (253.11% higher than CK). SOC and NH4+-N could explain 62% of the changes in the fungal community function. N addition can directly affect the bacterial community function or indirectly through NO3−-N. These results suggest that different microbial groups may have various responses to N addition. Compared with bacteria and fungi, the effect of N addition was less on the archaeal community. Meanwhile, N-mediated changes of the soil properties play an essential role in changes in the microbial community. The results in the present study provided a reliable basis for an understanding of how the microbial community in a desert ecosystem adapts to long-term N deposition.
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DOI：
10.1038/ismej.2011.159
发表时间：
2012-05
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.2
作者：
He J;Jiao S;Tan X;Wei H;Ma X;Nie Y;Liu J;Lu X;Mo J;Shen W
通讯作者：
Shen W
影响因子：
9.7
作者：
Freedman, Zachary B.;Romanowicz, Karl J.;Zak, Donald R.
通讯作者：
Zak, Donald R.
影响因子：
2.7
作者：
Han, Wen-Juan;Cao, Jia-Yu;Ni, Jian
通讯作者：
Ni, Jian
DOI：
10.1080/00103629109368455
发表时间：
1991-01-01
影响因子：
1.8
作者：
CIAVATTA, C;GOVI, M;SEQUI, P
通讯作者：
SEQUI, P