Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth.
Endoplasmic Reticulum-Localized Transmembrane Protein Dpy19L1 Is Required for Neurite Outgrowth.
复制标题
内质网 - 裂解跨膜蛋白DPY19L1是神经突生长所必需的。
DOI：
10.1371/journal.pone.0167985
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Takebayashi H
中科院分区：
文献类型：
--
作者：
Watanabe K;Bizen N;Sato N;Takebayashi H
内质网（ER），包括核膜，是一个连续和复杂的膜结合细胞器，负责各种细胞功能。在神经元中，ER网络存在于细胞体、轴突和树突中。最近的研究表明，ER网络参与神经元的发育，如神经元迁移和轴突生长。然而，ER定位蛋白对神经发育的调节还没有完全了解。我们以前报道，多跨膜蛋白Dpy 19 L1是必需的神经元迁移在发育中的小鼠大脑皮层。Dpy 19 L家族成员Dpy 19 L2是人类球形精子症的致病基因，被认为是顶体与核膜的锚。本研究中，我们发现外源性Dpy 19 L1在COS-7细胞中的表达模式与ER部分一致，包括在光学显微镜水平上的核膜。Dpy 19 L1的网状分布被微管解聚破坏，从而诱导ER的收缩。此外，Dpy 19 L1在胚胎小鼠皮层神经元中表现出与ER标记蛋白相似的分布模式。最后，我们表明，Dpy 19 L1敲低介导的siRNA导致减少神经突起生长在培养的神经元。这些结果表明，跨膜蛋白Dpy 19 L1是本地化的ER膜和调节神经突起的延伸过程中的发展。
The endoplasmic reticulum (ER), including the nuclear envelope, is a continuous and intricate membrane-bound organelle responsible for various cellular functions. In neurons, the ER network is found in cell bodies, axons, and dendrites. Recent studies indicate the involvement of the ER network in neuronal development, such as neuronal migration and axonal outgrowth. However, the regulation of neural development by ER-localized proteins is not fully understood. We previously reported that the multi-transmembrane protein Dpy19L1 is required for neuronal migration in the developing mouse cerebral cortex. A Dpy19L family member, Dpy19L2, which is a causative gene for human Globozoospermia, is suggested to act as an anchor of the acrosome to the nuclear envelope. In this study, we found that the patterns of exogenous Dpy19L1 were partially coincident with the ER, including the nuclear envelope in COS-7 cells at the level of the light microscope. The reticular distribution of Dpy19L1 was disrupted by microtubule depolymerization that induces retraction of the ER. Furthermore, Dpy19L1 showed a similar distribution pattern with a ER marker protein in embryonic mouse cortical neurons. Finally, we showed that Dpy19L1 knockdown mediated by siRNA resulted in decreased neurite outgrowth in cultured neurons. These results indicate that transmembrane protein Dpy19L1 is localized to the ER membrane and regulates neurite extension during development.
登录
查看更多内容
影响因子：
4.4
作者：
Carson, Andrew R.;Cheung, Joseph;Scherer, Stephen W.
通讯作者：
Scherer, Stephen W.
影响因子：
4.2
作者：
Aihara, Y;Inoue, T;Mikoshiba, K
通讯作者：
Mikoshiba, K
影响因子：
11.4
作者：
Klopfenstein, DRC;Kappeler, F;Hauri, HP
通讯作者：
Hauri, HP
影响因子：
9.8
作者：
Koscinski, Isabelle;Ellnati, Elias;Viville, Stephane
通讯作者：
Viville, Stephane
影响因子：
3.2
作者：
BROADWELL, RD;CATALDO, AM
通讯作者：
CATALDO, AM