Physical characterization and in vivo organ distribution of coated iron oxide nanoparticles.
Physical characterization and in vivo organ distribution of coated iron oxide nanoparticles.
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DOI：
10.1038/s41598-018-23317-2
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发表时间：
2018-03-20
影响因子：
4.6
通讯作者：
Ivkov R
中科院分区：
文献类型：
--
作者：
Sharma A;Cornejo C;Mihalic J;Geyh A;Bordelon DE;Korangath P;Westphal F;Gruettner C;Ivkov R
用羧甲基葡聚糖(CM-葡聚糖)、聚乙二醇聚乙二醇亚胺(PEG-PEI)、甲氧基-聚乙二醇磷酸盐+芦丁或葡聚糖中的一种对柠檬酸稳定的氧化铁磁性纳米颗粒(MNPs)进行包覆。对它们的大小、Zeta电位、在交变磁场中的滞后加热、动态磁化率进行了表征，并检查了它们在静脉注射后在小鼠器官中的分布。除聚乙二醇-聚乙二醇胺包覆纳米粒子外，所有包覆纳米粒子在生理pH下均具有负Zeta电位。通过动态光散射法测定纳米粒子的大小，发现纳米粒子在涂层上的流体力学直径增大。涂层对磁滞加热的影响不大，但较大颗粒的复合磁化率峰值明显向低频移动。在静脉注射纳米颗粒48 小时后，处死小鼠，收集组织测定铁浓度。具有负表面电位的纳米粒子的铁沉积被观察到在肝脏和脾中具有最高的积累量。相反，带正电的聚乙二醇PEI包裹的纳米粒子的铁沉积在肺部有最高的浓度。这些初步结果表明，纳米颗粒大小和电荷之间的复杂相互作用决定了系统传递的氧化铁磁性纳米颗粒的器官分布。
Citrate-stabilized iron oxide magnetic nanoparticles (MNPs) were coated with one of carboxymethyl dextran (CM-dextran), polyethylene glycol-polyethylene imine (PEG-PEI), methoxy-PEG-phosphate+rutin, or dextran. They were characterized for size, zeta potential, hysteresis heating in an alternating magnetic field, dynamic magnetic susceptibility, and examined for their distribution in mouse organs following intravenous delivery. Except for PEG-PEI-coated nanoparticles, all coated nanoparticles had a negative zeta potential at physiological pH. Nanoparticle sizing by dynamic light scattering revealed an increased nanoparticle hydrodynamic diameter upon coating. Magnetic hysteresis heating changed little with coating; however, the larger particles demonstrated significant shifts of the peak of complex magnetic susceptibility to lower frequency. 48 hours following intravenous injection of nanoparticles, mice were sacrificed and tissues were collected to measure iron concentration. Iron deposition from nanoparticles possessing a negative surface potential was observed to have highest accumulation in livers and spleens. In contrast, iron deposition from positively charged PEG-PEI-coated nanoparticles was observed to have highest concentration in lungs. These preliminary results suggest a complex interplay between nanoparticle size and charge determines organ distribution of systemically-delivered iron oxide magnetic nanoparticles.
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