Altering microtubule stability affects microtubule clearance and nuclear extrusion during erythropoiesis
Altering microtubule stability affects microtubule clearance and nuclear extrusion during erythropoiesis
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改变微管稳定性会影响红细胞生成过程中的微管清除和核挤出
DOI：
10.1002/jcp.28582
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发表时间：
2019-11
影响因子：
5.6
通讯作者：
Liu Min
中科院分区：
文献类型：
--
作者：
Xie Songbo;Yan Bing;Feng Jie;Wu Yuhan;He Na;Sun Lei;Zhou Jun;Li Dengwen;Liu Min
哺乳动物的红细胞是高度特化的细胞，在成熟过程中它们适应了失去细胞核及其他细胞成分，以确保氧气的输送。细胞核排出是红细胞生成过程中最关键的事件，它代表了一种极端情况的……（此处英文未完整表述，翻译到这里语义不完整）
Mammalian erythrocytes are highly specialized cells that have adapted to lose their nuclei and cellular components during maturation to ensure oxygen delivery. Nuclear extrusion, the most critical event during erythropoiesis, represents an extreme case of asymmetric partitioning that requires a dramatic reorganization of the cytoskeleton. However, the precise role of the microtubule cytoskeleton in the enucleation process remains controversial. In this study, we show that microtubule reorganization is critical for microtubule clearance and nuclear extrusion during erythropoiesis. Using a rodent anemia model, we found that microtubules were present in erythroblasts and reticulocytes but were undetectable in erythrocytes. Further analysis demonstrated that microtubules became disordered in reticulocytes and revealed that microtubule stabilization was critical for tubulin degradation. Disruption of microtubule dynamics using the microtubule‐stabilizing agent paclitaxel or the microtubule‐destabilizing agent nocodazole did not affect the efficiency of erythroblast enucleation. However, paclitaxel treatment resulted in the retention of tubulin in mature erythrocytes, and nocodazole treatment led to a defect in pyrenocyte morphology. Taken together, our data reveals a critical role for microtubules in erythrocyte development. Our findings also implicate the disruption of microtubule dynamics in the pathogenesis of anemia‐associated diseases, providing new insight into the pathogenesis of the microtubule‐targeted agent‐associated anemia frequently observed during cancer chemotherapy.
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影响因子：
3.7
作者：
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通讯作者：
Propst, Friedrich
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影响因子：
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作者：
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20.3
作者：
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J. Chasis;M. Prenant;A. Leung;N. Mohandas
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20.3
作者：
Konstantinidis, Diamantis G.;Pushkaran, Suvarnamala;Kalfa, Theodosia A.
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Kalfa, Theodosia A.