FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation.

FM reconstruction of non-uniformly sampled protein NMR data at higher dimensions and optimization by distillation.
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DOI:
10.1007/s10858-009-9368-1
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发表时间:
2009-11
影响因子:
2.7
通讯作者:
Wagner G
Wagner G
中科院分区:
生物学3区
文献类型:
--
作者:
Hyberts SG;Frueh DP;Arthanari H;Wagner G

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非均匀采样(NUS)能够以与现代仪器的分辨能力相匹配的分辨率记录多维NMR数据,而无需使用过多的测量时间。然而,为了获得令人满意的结果,需要有效的重建方法。在这里,我们描述了一个优化版本的前向最大熵(FM)重建方法,它可以重建多达三个间接的尺寸。对于复杂的数据集,如NOESY光谱,该程序的性能通过减少源于强峰的伪影的蒸馏程序来增强。
Non-uniform sampling (NUS) enables recording of multidimensional NMR data at resolutions matching the resolving power of modern instruments without using excessive measuring time. However, in order to obtain satisfying results, efficient reconstruction methods are needed. Here we describe an optimized version of the Forward Maximum entropy (FM) reconstruction method, which can reconstruct up to three indirect dimensions. For complex datasets, such as NOESY spectra, the performance of the procedure is enhanced by a distillation procedure that reduces artifacts stemming from intense peaks.
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