iTRAQ-Based Proteomics Analyses of Sterile/Fertile Anthers from a Thermo-Sensitive Cytoplasmic Male-Sterile Wheat with Aegilops kotschyi Cytoplasm.
iTRAQ-Based Proteomics Analyses of Sterile/Fertile Anthers from a Thermo-Sensitive Cytoplasmic Male-Sterile Wheat with Aegilops kotschyi Cytoplasm.
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基于 iTRAQ 对带有克氏山羊草细胞质的热敏细胞质雄性不育小麦的不育/可育花药进行蛋白质组学分析
DOI：
10.3390/ijms19051344
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发表时间：
2018-05-02
影响因子：
5.6
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Zhang G;Ye J;Jia Y;Zhang L;Song X
A “two-line hybrid system” was developed, previously based on thermo-sensitive cytoplasmic male sterility in Aegilops kotschyi (K-TCMS), which can be used in wheat breeding. The K-TCMS line exhibits complete male sterility and it can be used to produce hybrid wheat seeds during the normal wheat-growing season; it propagates via self-pollination at high temperatures. Isobaric tags for relative and absolute quantification-based quantitative proteome and bioinformatics analyses of the TCMS line KTM3315A were conducted under different fertility conditions to understand the mechanisms of fertility conversion in the pollen development stages. In total, 4639 proteins were identified, the differentially abundant proteins that increased/decreased in plants with differences in fertility were mainly involved with energy metabolism, starch and sucrose metabolism, phenylpropanoid biosynthesis, protein synthesis, translation, folding, and degradation. Compared with the sterile condition, many of the proteins that related to energy and phenylpropanoid metabolism increased during the anther development stage. Thus, we suggest that energy and phenylpropanoid metabolism pathways are important for fertility conversion in K-TCMS wheat. These findings provide valuable insights into the proteins involved with anther and pollen development, thereby, helping to further understand the mechanism of TCMS in wheat.
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基于 iTRAQ 的定量蛋白质组和磷蛋白表征揭示了小麦籽粒发育中涉及的中心代谢变化
影响因子：
4.4
作者：
Ma C;Zhou J;Chen G;Bian Y;Lv D;Li X;Wang Z;Yan Y
通讯作者：
Yan Y
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通讯作者：
Smalle, Jan A.
影响因子：
6.5
作者：
Han, Zongfu;Qin, Yuxiang;Li, Ruzhong
通讯作者：
Li, Ruzhong
影响因子：
11.6
作者：
Giegé, P;Heazlewood, JL;Sweetlove, LJ
通讯作者：
Sweetlove, LJ