Mechanism of cross-bridge detachment in isometric force relaxation of skeletal and cardiac myofibrils
Mechanism of cross-bridge detachment in isometric force relaxation of skeletal and cardiac myofibrils
复制标题
骨骼肌和心肌原纤维等长力松弛中跨桥脱离的机制
DOI：
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发表时间：
2004
影响因子：
2.7
通讯作者：
C. Tesi
中科院分区：
文献类型：
--
作者：
A. Belus;N. Piroddi;C. Tesi
骨骼肌和心肌松弛由两种大分子系统之间的相互作用控制：（i）膜结合的Ca 2+转运蛋白和（ii）肌节蛋白。光解实验在皮肤肌肉制剂和快速解决方案切换研究在单个肌原纤维提供的手段，分离肌节的松弛机制与肌浆Ca 2+去除。单肌原纤维实验最近表明，跨桥力学和脱离动力学是松弛时间过程的主要决定因素。肌原纤维中的全力衰减发生在两个阶段：一个缓慢的一个快速的。后者是由肌节'给'和肌节间的动力学为主，而前者发生在近等距条件下。强有力的证据已经发现，在肌原纤维松弛力衰减的速度慢，反映了在跨桥离开力产生状态下等距条件下的速度。对肌原纤维化学-力学转换过程的剖析表明，横桥从产生力状态到非产生力状态的正向和反向转换都有助于肌肉松弛。
Skeletal and cardiac muscle relaxation is governed by the interplay between two macromolecular systems: (i) membrane bound Ca2+ transport proteins and (ii) sarcomeric proteins. Photolysis experiments in skinned muscle preparations and fast solution switching studies in single myofibrils offer means for isolating sarcomeric mechanisms of relaxation from those related to myoplasmic Ca2+ removal. Single myofibril experiments have recently shown that cross-bridge mechanics and detachment kinetics are the major determinants of the time course of relaxation. Full force decay in myofibrils occurs in two phases: a slow one followed by a rapid one. The latter is initiated by sarcomere ‘give’ and dominated by inter-sarcomere dynamics while the former occurs under nearly isometric conditions. Strong evidence has been found that the slow rate of force decay in myofibril relaxation reflects the rate at which cross-bridges leave force-generating states under isometric conditions. Dissection of chemo-mechanical transduction process in myofibrils indicate that both forward and backward transitions of cross-bridges from force-generating to non-force-generating states contribute to muscle relaxation.
肌球蛋白调节轻链的磷酸化消除了骨骼肌松弛率的力依赖性变化。
DOI：
10.1016/s0006-3495(98)77793-8
发表时间：
1998
期刊：
Biophysical journal.
影响因子：
--
作者：
Patel,JR;Diffee,GM;Huang,XP;Moss,RL
通讯作者：
Moss,RL
去皮青蛙骨骼肌纤维松弛率的决定因素。
DOI：
10.1152/ajpcell.1998.274.6.c1608
发表时间：
1998
期刊：
The American journal of physiology
影响因子：
--
作者：
Wahr,PA;Johnson,JD;Rall,JA
通讯作者：
Rall,JA