Biomanufacturing of value‐added products from oils or fats: A case study on cellular and fermentation engineering of Yarrowia lipolytica
Biomanufacturing of value‐added products from oils or fats: A case study on cellular and fermentation engineering of Yarrowia lipolytica
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利用油脂生物制造增值产品：解脂耶氏酵母细胞和发酵工程案例研究
DOI：
10.1002/bit.27685
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发表时间：
2021
影响因子：
3.8
通讯作者：
Xie, Dongming
中科院分区：
文献类型：
--
作者：
Liu, Na;Soong, Ya‐Hue V.;Mirzaee, Iman;Olsen, Andrew;Yu, Peng;Wong, Hsi‐Wu;Xie, Dongming
美国每年产生1000多万吨废弃油脂。本文旨在建立一个新的生物制造平台，将废弃油脂转化为一系列增值产品。本研究以产油酵母解脂耶氏酵母（Yarrowia lipolytica）为研究对象，以废油脂为原料生产柠檬酸.首先，我们进行了计算流体动力学（CFD）模拟的生物反应器系统，并确定了胞外混合和传质是第一个限制因素的油脂发酵过程中，由于在水中的不溶性油。基于CFD模拟结果，优化了生物反应器的设计和操作条件，并在补料分批发酵实验中成功地提高了油的吸收和生物转化。在此之后，我们通过在野生型Y中过表达和缺失MHY1基因来研究细胞形态对油摄取、细胞内脂质积累和CA形成的影响。解脂剂ATCC 20362。细胞形态与生物量、脂肪和CA生产率之间具有良好的线性相关性（R2> 0.82）。最后，比较了葡萄糖和油底物的发酵动力学，并仔细评估了油发酵过程。我们的研究表明，废弃油脂可以成为许多高价值产品生物制造的经济原料。
The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that converts waste oils or fats into a series of value‐added products. Our research employs the oleaginous yeastYarrowia lipolyticaas a case study for citric acid (CA) production from waste oils. First, we conducted the computational fluid dynamics (CFD) simulation of the bioreactor system and identified that the extracellular mixing and mass transfer is the first limiting factor of an oil fermentation process due to the insolubility of oil in water. Based on the CFD simulation results, the bioreactor design and operating conditions were optimized and successfully enhanced oil uptake and bioconversion in fed‐batch fermentation experiments. After that, we investigated the impacts of cell morphology on oil uptake, intracellular lipid accumulation, and CA formation by overexpressing and deleting theMHY1gene in the wild typeY. lipolyticaATCC20362. Fairly good linear correlations (R2> 0.82) were achieved between cell morphology and productivities of biomass, lipid, and CA. Finally, fermentation kinetics with both glucose and oil substrates were compared and the oil fermentation process was carefully evaluated. Our study suggests that waste oils or fats can be economical feedstocks for biomanufacturing of many high‐value products.
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用于将植物油生物转化为高价值产品的新型生物制造平台
DOI：
--
发表时间：
2018
期刊：
影响因子：
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作者：
D. Xie;Y. Soong;Na Liu;Jian;Steven Chen;J. Keats;Iman Mirzaee;C. Lawton
通讯作者：
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影响因子：
2.9
作者：
J. Sarkar;L. K. Shekhawat;V. Loomba;A. Rathore
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A. Rathore
影响因子：
2.9
作者：
Lucatero, S;Larralde-Corona, CP;Galindo, E
通讯作者：
Galindo, E
影响因子：
3.9
作者：
Liu, Na;Liu, Bo;Xie, Dongming
通讯作者：
Xie, Dongming
影响因子：
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作者：
Lee, N;Kronstad, JW
通讯作者：
Kronstad, JW