Purified Protein Delivery to Activate an Epigenetically Silenced Allele in Mouse Brain.
Purified Protein Delivery to Activate an Epigenetically Silenced Allele in Mouse Brain.
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纯化的蛋白质递送可激活小鼠大脑中的表观遗传沉默等位基因。
DOI：
10.1007/978-1-4939-7774-1_12
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Segal,DavidJ
中科院分区：
文献类型：
--
作者：
Pyles,Benjamin;Bailus,BarbaraJ;O'Geen,Henriette;Segal,DavidJ
The ability to activate or repress specific genes in the brain could have a tremendous impact for understanding and treating neurological disorders. Artificial transcription factors based on zinc finger, TALE, and CRISPR/Cas9 programmable DNA-binding platforms have been widely used to regulate the expression of specific genes in cultured cells, but their delivery into the brain represents a critical challenge to apply such tools in live animals. In previous work, we developed a purified, zinc finger-based artificial transcription factor that could be injected systemically, cross the blood-brain barrier, and alter expression of a specific gene in the brain of an adult mouse model of Angelman syndrome. Importantly, our mode of delivery produced widespread distribution throughout the brain. Here we describe our most current methods for the production and purification of the factor, dosage optimization, and use of live animal fluorescence imaging to visualize the kinetics of distribution.
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影响因子：
12.4
作者：
Cearley, CN;Wolfe, JH
通讯作者：
Wolfe, JH
DOI：
10.1007/978-1-4939-2932-0_7
发表时间：
2016
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Thakore PI;Gersbach CA
通讯作者：
Gersbach CA
影响因子：
46.9
作者：
Deverman, Benjamin E.;Pravdo, Piers L.;Simpson, Bryan P.;Kumar, Sripriya Ravindra;Chan, Ken Y.;Banerjee, Abhik;Wu, Wei-Li;Yang, Bin;Huber, Nina;Pasca, Sergiu P.;Gradinaru, Viviana
通讯作者：
Gradinaru, Viviana
影响因子：
4
作者：
Gaj T;Liu J;Anderson KE;Sirk SJ;Barbas CF 3rd
通讯作者：
Barbas CF 3rd
影响因子：
12.4
作者：
Gray, Steven J.;Matagne, Valerie;Samulski, R. Jude
通讯作者：
Samulski, R. Jude