Identification of maize silicon influx transporters.
Identification of maize silicon influx transporters.
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DOI：
10.1093/pcp/pcn110
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发表时间：
2009-01
影响因子：
4.9
通讯作者：
Ma JF
中科院分区：
文献类型：
--
作者：
Mitani N;Yamaji N;Ma JF
玉米（Zea mays L.）显示了硅（Si）的高积累，但尚未确定参与摄取和分布的转运蛋白。在本研究中，我们分离到两个基因（ZmLsi1和ZmLsi6），这是同源的水稻硅内流转运蛋白OsLsi1。在非洲爪蟾卵母细胞中的异源表达表明ZmLsi1和ZmLsi6都是可渗透的。ZmLsi1主要在根中表达。相比之下，ZmLsi6在叶鞘和叶片中表达更多。与OsLsi1不同，ZmLsi1和ZmLsi6的表达水平不受Si供应的影响。免疫组织化学染色结果表明，ZmLsi1定位于种子根和冠根根表皮细胞和下皮层细胞远端侧的质膜上，侧根皮层细胞中也有ZmLsi1的表达。在芽中，在叶鞘和叶片中与导管相邻的木质部薄壁细胞中发现ZmLsi6。叶鞘和叶片中的ZmLsi6也表现出极性定位在面向容器的一侧。综上所述，可以得出结论，ZmLsi1是一个内流运输的Si，这是负责运输的Si从外部溶液到根细胞和ZmLsi6主要功能作为一个Si转运木质部卸载。
Maize (Zea mays L.) shows a high accumulation of silicon (Si), but transporters involved in the uptake and distribution have not been identified. In the present study, we isolated two genes (ZmLsi1 and ZmLsi6), which are homologous to rice influx Si transporter OsLsi1. Heterologous expression in Xenopus laevis oocytes showed that both ZmLsi1 and ZmLsi6 are permeable to silicic acid. ZmLsi1 was mainly expressed in the roots. By contrast, ZmLsi6 was expressed more in the leaf sheaths and blades. Different from OsLsi1, the expression level of both ZmLsi1 and ZmLsi6 was unaffected by Si supply. Immunostaining showed that ZmLsi1 was localized on the plasma membrane of the distal side of root epidermal and hypodermal cells in the seminal and crown roots, and also in cortex cells in lateral roots. In the shoots, ZmLsi6 was found in the xylem parenchyma cells that are adjacent to the vessels in both leaf sheaths and leaf blades. ZmLsi6 in the leaf sheaths and blades also exhibited polar localization on the side facing towards the vessel. Taken together, it can be concluded that ZmLsi1 is an influx transporter of Si, which is responsible for the transport of Si from the external solution to the root cells and that ZmLsi6 mainly functions as a Si transporter for xylem unloading.
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DOI：
10.1073/pnas.0606330103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Fauteux, Francois;Chain, Florian;Belanger, Richard R.
通讯作者：
Belanger, Richard R.
影响因子：
5.1
作者：
Hachez, Charles;Moshelion, Menachem;Chaumont, Francois
通讯作者：
Chaumont, Francois
影响因子：
7.4
作者：
Chaumont, F;Barrieu, F;Jung, R
通讯作者：
Jung, R
影响因子：
9.4
作者：
Liang, Yongchao;Hua, Haixia;Roemheld, Volker
通讯作者：
Roemheld, Volker
影响因子：
7.4
作者：
Ma, JF;Goto, S;Ichii, M
通讯作者：
Ichii, M