MicroRNA-23a promotes neuroblastoma cell metastasis by targeting CDH1.
MicroRNA-23a promotes neuroblastoma cell metastasis by targeting CDH1.
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DOI：
10.3892/ol.2014.1794
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发表时间：
2014-03
期刊：
影响因子：
2.9
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Cheng L;Yang T;Kuang Y;Kong B;Yu S;Shu H;Zhou H;Gu J
CDH 1失活在肿瘤转移中是重要的。在本研究中，它被认为是CDH 1的mRNA和蛋白水平的下降，在转移性神经母细胞瘤（NB）组织相比，在那些在原发NB组织。本研究的目的是探讨转移性NB中CDH 1下调的调控机制。MicroRNA是小的非编码RNA（长度约22 nt），其负调节靶mRNA并参与各种癌症相关过程，包括转移。在当前的研究中，与原发性NB组织相比，miR-23 a在人转移性NB组织中显示出上调。抑制miR-23 a可显著抑制NB细胞的迁移和侵袭。体外报告基因分析表明，CDH 1是miR-23 a的直接靶基因。阻断miR-23 a的表达可部分恢复NB细胞中CDH 1的表达。这些发现为miR-23 a通过靶向CDH 1促进NB细胞迁移和侵袭提供了证据，并提示外源性miR-23 a可能在治疗NB转移中具有治疗价值。
CDH1 inactivation is important in tumor metastasis. In the present study, it was suggested that the mRNA and protein levels of CDH1 decreased in metastatic neuroblastoma (NB) tissues compared with those in primary NB tissues. The aim of the study was to explore the regulatory mechanisms of CDH1 downregulation in metastatic NB. MicroRNAs are small non-coding RNAs (~22 nt in length) that negatively regulate target mRNAs and are involved in various cancer-related processes, including metastasis. In the current study, miR-23a was shown to be upregulated in human metastatic NB tissues compared with primary NB tissues. Inhibition of miR-23a may significantly suppress NB cell migration and invasion. In vitro reporter assay suggested that CDH1 is a direct target gene of miR-23a. Furthermore, blocking the expression of miR-23a partly restored the expression of CDH1 in NB cells. These findings provide evidence that miR-23a is key in promoting NB cell migration and invasion through targeting CDH1, and suggest that exogenous miR-23a may have therapeutic value in treating NB metastasis.
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影响因子：
11.2
作者：
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通讯作者：
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DOI：
10.1073/pnas.0500918102
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