In Vivo Confocal Microscopy Shows Alterations in Nerve Density and Dendritiform Cell Density in Fuchs' Endothelial Corneal Dystrophy.
In Vivo Confocal Microscopy Shows Alterations in Nerve Density and Dendritiform Cell Density in Fuchs' Endothelial Corneal Dystrophy.
复制标题
DOI：
10.1016/j.ajo.2018.08.040
复制
发表时间：
2018-12
影响因子：
4.2
通讯作者：
Hamrah P
中科院分区：
文献类型：
--
作者：
Aggarwal S;Cavalcanti BM;Regali L;Cruzat A;Trinidad M;Williams C;Jurkunas UV;Hamrah P
通过激光体内共聚焦显微镜（IVCM）评价Fuchs角膜内皮营养不良（FECD）和人工晶状体大泡性角膜病变（PBK）的角膜神经和免疫细胞改变与感觉和内皮细胞丢失的相关性。前瞻性、横断面、对照研究。在三级转诊中心，将33只FECD眼与13只PBK眼和17只正常年龄匹配的对照眼进行比较。FECD分为早期（无水肿）和晚期（有水肿）。进行角膜IVCM（HRT 3/RCM）和感觉测量（Cochet-Bonnet）。角膜神经和免疫树突状细胞（DC）的变化进行了评估，并与临床参数。与对照组（23.3±8.1和25.9±1.3）相比，FECD和PBK眼显示总神经长度（11.5±1.3和2.9± 0.7 mm/mm 2）和数量（8.8±1.1和2.2±0.4 n/帧）显著减少（p=0.001）。与对照组（5.9 ±0.04）相比，神经减少对应于FECD感觉减少（4.9±0.2cm; R=0.32; p=0.045）。与对照组相比，早期和晚期FECD显示总神经长度（分别为13.1±1.4和9.9±1.2 mm/mm 2）和数量（8.2±2.5和6.5±2.1 n/帧）显著减少（p<0.001）。与对照组（22.5±4.5）相比，FECD（57.8±10.4个细胞/mm 2; p=0.01）中的DC密度显著增加，但在PBK（47.7±11.6; p=0.60）中未显著增加。早期FECD患者的一个子集（7/22）表现出非常高的DC密度（>100/mm 2）。IVCM显示，在早期、晚期FECD和PBK中，基底下角膜神经显著减少，与感觉降低相关。早期FECD中增加的DC密度表明潜在的亚临床炎症。这些数据表明，减少基底神经和增加免疫激活可能发挥作用的病理生理学FECD。
To evaluate corneal nerve and immune cell alterations in Fuchs’ endothelial corneal dystrophy (FECD) and pseudophakic bullous keratopathy (PBK) by laser in vivo confocal microscopy (IVCM) as correlated to sensation and endothelial cell loss. Prospective, cross-sectional, controlled study. 33 eyes with FECD were compared to 13 eyes with PBK and 17 normal age-matched control eyes at a tertiary referral center. FECD was classified into early (without edema) and late stage (with edema). Corneal IVCM (HRT3/RCM) and esthesiometry (Cochet-Bonnet) were performed. Corneal nerve and immune dendritic cell (DC) alterations were evaluated and correlated to clinical parameters. FECD and PBK eyes showed significantly (p=0.001) diminished total nerve length (11.5±1.3 and 2.9±0.7mm/mm2) and number (8.8±1.1 and 2.2±0.4 n/frame), compared to controls (23.3±8.1 and 25.9±1.3). Decreased nerves corresponded to diminished sensation in FECD (4.9±0.2cm; R=0.32; p=0.045), compared to controls (5.9±0.04). Early and late stage FECD showed significantly reduced total nerve length (13.1±1.4 and 9.9±1.2 mm/mm2, respectively) and number (8.2±2.5 and 6.5±2.1 n/frame), compared to controls (p<0.001). DC density was significantly increased in FECD (57.8±10.4 cells/mm2; p=0.01), but not in PBK (47.7±11.6; p=0.60) compared to controls (22.5±4.5). A subset of early FECD patients (7/22) demonstrated very high DC density (>100/mm2). IVCM demonstrates profound diminishment of subbasal corneal nerves in early, late stage FECD, and PBK, correlating to decreased sensation. Increased DC density in early FECD demonstrates potential subclinical inflammation. The data suggest that reduction in subbasal nerves and increased immune activation may play a role in pathophysiology of FECD.
登录
查看更多内容
影响因子：
6.4
作者：
Elhalis, Hussain;Azizi, Behrooz;Jurkunas, Ula V.
通讯作者：
Jurkunas, Ula V.
影响因子：
1.6
作者：
Ghosheh, Faris R.;Cremona, Federico A.;Laibson, Peter R.
通讯作者：
Laibson, Peter R.
影响因子：
4.4
作者：
Koh, Shay-Whey M.;Chandrasekara, Krish;Rutzen, Allan R.
通讯作者：
Rutzen, Allan R.
影响因子：
64.5
作者：
Cisse, Babacar;Caton, Michele L.;Lehner, Manfred;Maeda, Takahiro;Scheu, Stefanie;Locksley, Richard;Holmberg, Dan;Zweier, Christiane;den Hollander, Nicolette S.;Kant, Sarina G.;Holter, Wolfgang;Rauch, Anita;Zhuang, Yuan;Reizis, Boris
通讯作者：
Reizis, Boris
影响因子：
13.7
作者：
Hamrah P;Cruzat A;Dastjerdi MH;Zheng L;Shahatit BM;Bayhan HA;Dana R;Pavan-Langston D
通讯作者：
Pavan-Langston D