Morphology of the yeast endocytic pathway.
Morphology of the yeast endocytic pathway.
复制标题
酵母内吞途径的形态学。
DOI：
10.1091/mbc.9.1.173
复制
发表时间：
1998
影响因子：
3.3
通讯作者：
H. Riezman
中科院分区：
文献类型：
--
作者：
C. Prescianotto‐Baschong;H. Riezman
带正电荷的 Nanogold（Nanoprobes，Stony Brook，NY）已被开发为追踪酵母内吞途径的新标记物。带正电荷的纳米金广泛结合到酵母原生质球的表面，并以能量依赖性方式内化。 end3 突变体中金的内化被阻断。在15℃下将酵母原生质球与带正电荷的纳米金一起孵育的过程中，在小囊泡、我们称之为早期内体的外周囊泡/管状区室、与液泡附近的晚期内体相对应的多囊泡体以及液泡中依次检测到金。检查 sec18 突变体内吞作用的实验表明，带正电荷的 Nanogold 在直径约 30-50 nm 的囊泡中积累。这些囊泡很可能代表初级内吞囊泡，因为在突变细胞中没有检测到其他中间体，并且它们的大小与在 15 摄氏度下在野生型原生质球中检测到的第一个囊泡相对应。这些数据有力地支持了这样的观点，即酵母中内吞作用的内化步骤涉及从质膜形成大小均匀的小囊泡。
Positively charged Nanogold (Nanoprobes, Stony Brook, NY) has been developed as a new marker to follow the endocytic pathway in yeast. Positively charged Nanogold binds extensively to the surface of yeast spheroplasts and is internalized in an energy-dependent manner. Internalization of gold is blocked in the end3 mutant. During a time course of incubation of yeast spheroplasts with positively charged Nanogold at 15 degrees C, the gold was detected sequentially in small vesicles, a peripheral, vesicular/tubular compartment that we designate as an early endosome, a multivesicular body corresponding to the late endosome near the vacuole, and in the vacuole. Experiments examining endocytosis in the sec18 mutant showed an accumulation of positively charged Nanogold in approximately 30-50 nm diameter vesicles. These vesicles most likely represent the primary endocytic vesicles as no other intermediates were detected in the mutant cells, and they correspond in size to the first vesicles detected in wild-type spheroplasts at 15 degrees C. These data lend strong support to the idea that the internalization step of endocytosis in yeast involves formation of small vesicles of uniform size from the plasma membrane.
影响因子：
4
作者：
F. Schimmöller;H. Riezman
通讯作者：
F. Schimmöller;H. Riezman