Coexistence of galanin-like immunoreactivity with catecholamines, 5- hydroxytryptamine, GABA and neuropeptides in the rat CNS
Coexistence of galanin-like immunoreactivity with catecholamines, 5- hydroxytryptamine, GABA and neuropeptides in the rat CNS
复制标题
大鼠中枢神经系统中甘丙肽样免疫反应与儿茶酚胺、5-羟色胺、GABA 和神经肽的共存
DOI：
10.1523/jneurosci.06-12-03640.1986
复制
发表时间：
1986
影响因子：
3.8
通讯作者：
M. Goldstein
中科院分区：
文献类型：
--
作者：
T. Melander;T. Hökfelt;Åke Rökaeus;A. C. Cuello;W. H. Oertel;A. Verhofstad;M. Goldstein
通过相邻切片、免疫细胞化学和免疫细胞化学的双重标记，绘制了大鼠中枢神经系统中甘丙氨酸（GAL）样免疫反应性（LI）与儿茶酚胺、5-羟色胺（5-HT）、GABA或某些神经肽标记物共存的图谱。观察到许多共存的例子，但也有许多gal阳性的细胞体群体显示出与这些标记相似的分布，但没有明显的共存。在下丘脑弓形核中，酪氨酸羟化酶（TH）阳性细胞体（A12细胞）中均有大量GAL-LI存在于背内侧和腹外侧亚区，后者数量较多。在弓形核后侧面谷氨酸脱羧酶（GAD）阳性的体中，以及在正中隆起外层纤维的所有背侧水平上，GAL-LI共存。在下丘脑前部，大量的旁细胞和大细胞室旁核细胞含有GAL-LI和抗利尿素- li。此外，含有GAD-和GAL-LI的体在结节和尾端大细胞核的乳头隐窝外侧可见。用逆行示踪和免疫荧光相结合的方法显示，尾侧组的部分神经元投射到枕叶皮层。在中脑和髓质儿茶酚胺神经元中，GAL-LI在去肾上腺素能体蓝斑（A6细胞组）和第四脑室背侧的A4组以及迷走背复合体A2组的尾侧部分中有很大比例共存。然而，在后者的更多吻侧部分，特别是在孤立束核的内侧细分，可以看到大量GAL-IR小细胞体与儿茶酚胺神经元混杂在一起，但它们不含TH-LI。此外，GAL-IR细胞体与TH-IR体共展，但不共存，在C1组腹外侧髓质的C1（肾上腺素）孔中，在残肢区水平和C1组的最吻侧可见。最后，中脑和中缝髓核的5-HT阳性细胞体以及海马中粗5-HT神经纤维亚群共同含有GAL-LI。目前的结果表明，gal样肽存在于许多含有其他神经活性化合物的系统中，包括多巴胺、去甲肾上腺素、5-羟色胺、GABA和抗利尿激素。（摘要删节为400字）
The coexistence of galanin (GAL)-like immunoreactivity (LI) with markers for catecholamines, 5-hydroxytryptamine (5-HT), GABA, or some neuropeptides was mapped in the rat CNS by using adjacent sections, as well as by elution-restaining and double-labeling immunocytochemistry. Many instances of coexistence were observed, but there were also numerous GAL-positive cell body populations displaying distributions similar to those of these markers but without apparent coexistence. In the hypothalamic arcuate nucleus GAL-LI was found in a large proportion of tyrosine hydroxylase (TH)-positive cell bodies (A12 cells), both in the dorsomedial and ventrolateral subdivisions, with a higher number in the latter. GAL-LI coexisted in glutamic acid decarboxylase (GAD)- positive somata in the posterior aspects of the arcuate nucleus and at all rostrocaudal levels in fibers in the external layer of the median eminence. In the anterior hypothalamus, a large population of the cells of the parvocellular and magnocellular paraventricular nuclei contained both GAL-LI and vasopressin-LI. Moreover, somata containing both GAD- and GAL-LI were seen lateral to the mammillary recess in the tuberal and caudal magnocellular nuclei. Some of the neurons of the caudal group were shown to project to the occipital cortex using combined retrograde tracing and immunofluorescence. With regard to mesencephalic and medullary catecholamine neurons, GAL-LI coexisted in a large proportion of the noradrenergic locus coeruleus somata (A6 cell group) and in the A4 group dorsolateral to the fourth ventricle, as well as in the caudal parts of the A2 group in the dorsal vagal complex. However, in more rostral parts of the latter, especially in the medial subdivision of the solitary tract nucleus, a very large population of GAL-IR small cell bodies was seen intermingling with catecholamine neurons, but they did not contain TH-LI. Furthermore, GAL-IR cell bodies coextensive with, but not coexisting in, TH-IR somata were seen in the C1 (epinephrine) horea in the ventrolateral medulla at the level of area postrema and in the most rostral aspects of the C1 group. Finally, 5-HT-positive cell bodies of the mesencephalic and medullary raphe nuclei and a subpopulation of coarse 5-HT nerve fibers in the hippocampus co-contained GAL-LI. The present results demonstrate that a GAL-like peptide is present in many systems containing other neuroactive compounds, including dopamine, norepinephrine, 5-HT, GABA, and vasopressin.(ABSTRACT TRUNCATED AT 400 WORDS)
影响因子：
56.9
作者：
J. Nagy;L. LaBella;M. Buss;P. Daddona
通讯作者：
J. Nagy;L. LaBella;M. Buss;P. Daddona
对苯二胺可延缓免疫荧光的褪色并增强其强度。
DOI：
10.1177/31.6.6341464
发表时间：
1983
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Platt,JL;Michael,AF
通讯作者：
Michael,AF