MATH-2, a mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal, is specifically expressed in the nervous system.
MATH-2, a mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal, is specifically expressed in the nervous system.
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MATH-2是一种哺乳动物螺旋-环-螺旋因子，其结构与果蝇原神经基因无调性产物相关，在神经系统中特异性表达。
DOI：
10.1111/j.1432-1033.1995.0239l.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ryoichiro Kageyama
中科院分区：
文献类型：
--
作者：
Chikara Shevlizu;C. Akazawa;S. Nakanishi;Ryoichiro Kageyama
在果蝇中，多螺旋-环-螺旋（HLH）因子在神经发育中起重要作用。这些果蝇HLH因子的哺乳动物同源物最近已被鉴定，并为哺乳动物神经发生机制的分析提供了有用的信息。我们在这里报告的分子特征的一种新的小鼠HLH因子，指定MATH-2，具有结构同源性的果蝇原神经基因的产物atonal。MATH-2由337个氨基酸残基组成，含有一个与无调性相关的碱性HLH结构域。然而，在这个域之外，MATH-2和Atonal之间没有显著的序列相似性。MATH-2表达发生在胚胎第11.5天（E11.5），并且首先在脑囊泡壁以及脊髓中检测到。它在整个发育中的中枢神经系统的皮质板和套层中表达，但不在脑室区中表达。到E13.5，在大脑皮质板中的表达变得更加突出，但在其他区域中的表达减少。在成人中，大脑产生高水平的MATH-2 RNA，但其他神经组织仅产生低水平。在非神经组织中未检测到MATH-2 RNA，表明MATH-2表达对神经系统具有特异性。凝胶迁移率变化分析表明，MATH-2可以与E47（一种普遍表达的HLH因子）合作与几个E-box序列相互作用。这些结果提出了MATH-2可能是哺乳动物神经系统发育和维持中的反式作用因子的可能性。
In Drosophila, multiple helix-loop-helix (HLH) factors play an essential role in neural development. Mammalian homologues of such Drosophila HLH factors have been recently characterized and provide useful information for the analysis of the mechanisms of mammalian neurogenesis. We report here the molecular characterization of a novel mouse HLH factor, designated MATH-2, that has a structural homology to the product of Drosophila proneural gene atonal. MATH-2 consists of 337 amino acid residues and contains an atonal-related basic HLH domain. However, outside of this domain, there is no significant sequence similarity between MATH-2 and Atonal. MATH-2 expression occurs by embryonic day 11.5 (E11.5), and is first detected in the wall of brain vesicles as well as in the spinal cord. It is expressed in the cortical plate and the mantle layer throughout the developing central nervous system but not in the ventricular zone. By E13.5, the expression becomes more prominent in the cortical plate of the cerebrum but decreases in the other regions. In the adult, the cerebrum produces a high level of MATH-2 RNA but other neural tissues produce only low levels. MATH-2 RNA is not detected in non-neural tissues, indicating that MATH-2 expression is specific to the nervous system. The gel mobility-shift analysis shows that MATH-2 can interact with several E-box sequences in collaboration with E47, a ubiquitously expressed HLH factor. These results raise the possibility that MATH-2 may be a trans-acting factor involved in the development and maintenance of the mammalian nervous system.
DOI：
10.1073/pnas.88.5.1815
发表时间：
1991-03-01
影响因子：
11.1
作者：
CHRISTY, BA;SANDERS, LK;NATHANS, D
通讯作者：
NATHANS, D
影响因子：
4.6
作者：
K. Zimmerman;J. Shih;J. Bars;A. Collazo;D. Anderson
通讯作者：
K. Zimmerman;J. Shih;J. Bars;A. Collazo;D. Anderson