Effectiveness of Self-Collected, Ambient Temperature-Preserved Nasal Swabs Compared to Samples Collected by Trained Staff for Genotyping of Respiratory Viruses by Shotgun RNA Sequencing: Comparative Study.
Effectiveness of Self-Collected, Ambient Temperature-Preserved Nasal Swabs Compared to Samples Collected by Trained Staff for Genotyping of Respiratory Viruses by Shotgun RNA Sequencing: Comparative Study.
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DOI：
10.2196/32848
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发表时间：
2023-11-24
影响因子：
2.2
通讯作者：
中科院分区：
文献类型：
--
作者：
SARS-CoV-2 大流行凸显了现场标本采集和运输至诊断和公共卫生实验室的必要性。不依赖冷链运输的自行采集鼻拭子有可能消除检测的关键障碍，扩大检测能力，并减少卫生专业人员在大流行背景下暴露的机会。我们将研究参与者自己及其孩子在家中采集的鼻拭子与训练有素的研究人员采集的鼻拭子进行了比较。每位成年参与者收集 1 个鼻拭子，用单个拭子对两个鼻孔进行采样，然后从 1 名儿童收集 1 个鼻拭子。在收集了该家庭的所有参与者样本后，研究人员从每个人那里收集了一个单独的重复样本。样本采集后，成人参与者立即完成了一份有关抽样程序可接受性的调查问卷。将拭子放入温度稳定的防腐剂中，通过鸟枪法 RNA 测序检测呼吸道病毒，从而实现病毒基因组分析。总共有 21 个家庭参与了这项研究，每户有 1 名成人和 1 名儿童，共产生 42 名配对样本。研究参与者表示，自行收集是可以接受的。通过 RNA 测序在两个拭子中鉴定出的呼吸道病毒之间的一致性表明，通过简短的说明即可实现足够的收集技术。我们的结果支持了一种可扩展且方便的方法来识别呼吸道病毒以及实施新型呼吸道病原体大流行防范的可行性。
The SARS-CoV-2 pandemic has underscored the need for field specimen collection and transport to diagnostic and public health laboratories. Self-collected nasal swabs transported without dependency on a cold chain have the potential to remove critical barriers to testing, expand testing capacity, and reduce opportunities for exposure of health professionals in the context of a pandemic. We compared nasal swab collection by study participants from themselves and their children at home to collection by trained research staff. Each adult participant collected 1 nasal swab, sampling both nares with the single swab, after which they collected 1 nasal swab from 1 child. After all the participant samples were collected for the household, the research staff member collected a separate single duplicate sample from each individual. Immediately after the sample collection, the adult participants completed a questionnaire about the acceptability of the sampling procedures. Swabs were placed in temperature-stable preservative and respiratory viruses were detected by shotgun RNA sequencing, enabling viral genome analysis. In total, 21 households participated in the study, each with 1 adult and 1 child, yielding 42 individuals with paired samples. Study participants reported that self-collection was acceptable. Agreement between identified respiratory viruses in both swabs by RNA sequencing demonstrated that adequate collection technique was achieved by brief instructions. Our results support the feasibility of a scalable and convenient means for the identification of respiratory viruses and implementation in pandemic preparedness for novel respiratory pathogens.
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影响因子：
4.4
作者：
Malosh RE;Petrie JG;Callear AP;Monto AS;Martin ET
通讯作者：
Martin ET
影响因子：
3.2
作者：
Emerson, Julia;Cochrane, Elizabeth;Campbell, Angela P.
通讯作者：
Campbell, Angela P.
影响因子：
4.8
作者：
Ricci S;Lodi L;Citera F;Nieddu F;Moriondo M;Guarnieri V;Giovannini M;Indolfi G;Resti M;Zanobini A;Azzari C
通讯作者：
Azzari C
影响因子：
4
作者：
Muñoz-Ramírez S;Escribano-López B;Rodrigo-Casares V;Vergara-Hernández C;Gil-Mary D;Sorribes-Monrabal I;Garcés-Sánchez M;Muñoz-Del-Barrio MJ;Albors-Fernández AM;Úbeda-Sansano MI;Planelles-Cantarino MV;Largo-Blanco EM;Suárez-Vicent E;García-Rubio J;Bruijning-Verhagen P;Orrico-Sánchez A;Díez-Domingo J
通讯作者：
Díez-Domingo J
影响因子：
4.6
作者：
Li, Ci-Xiu;Li, Wei;Shi, Mang
通讯作者：
Shi, Mang