Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study.
Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study.
复制标题
在家庭流感疫苗评价研究中家庭采集鼻拭子检测流感。
DOI：
10.1111/irv.12822
复制
发表时间：
2021-03
影响因子：
4.4
通讯作者：
Martin ET
中科院分区：
文献类型：
--
作者：
Malosh RE;Petrie JG;Callear AP;Monto AS;Martin ET
基于社区的流感和其他呼吸道病毒（例如 SARS-CoV-2）研究需要实验室确认感染。在当前的 COVID-19 大流行期间，社交距离准则需要替代数据收集，以保护研究人员和参与者。家庭收集的呼吸道标本资源密集度较低，可以在症状出现后更早收集，并提供低接触的数据收集方式。前瞻性、多年性、基于社区的队列研究是检验家庭采集的标本在流感鉴定中的效用的理想环境。我们描述了家庭采集的标本用于检测流感的可行性和可靠性。我们从 2014 年 10 月至 2017 年 6 月期间的家庭流感疫苗评估 (HIVE) 研究中收集了数据和样本。队列参与者被要求在急性呼吸道疾病发作时在家中收集鼻拭子。研究人员还在发病后 7 天内在研究诊所收集了鼻子和喉咙拭子标本。我们使用 Cohen's kappa 估计了一致性，并计算了家庭采集的样本与工作人员采集的样本相比的敏感性和特异性。我们通过 RT-PCR 对 336 份工作人员和家庭收集的呼吸道样本进行了流感检测；工作人员采集的 150 份样本呈甲型/H3N2 流感阳性，23 份甲型/H1N1 流感阳性，14 份乙型流感/维多利亚样本，31 份乙型流感/山形流感阳性。我们发现 A/H3N2 流感 (0.70) 和 B/Yamagata (0.69) 的收集方法之间具有中等一致性，而 A/H1N1 流感 (0.87) 和 B/Victoria (0.86) 的收集方法具有高度一致性。所有流感类型和亚型的敏感性范围为 78% 至 86%。 A/H1N1 流感和两种 B 型流感谱系的特异性较高，范围为 96% 至 100%，而 A/H3N2 感染的特异性稍低 (88%)。在家采集鼻拭子标本对于鉴定流感病毒感染既可行又可靠。
Community‐based studies of influenza and other respiratory viruses (eg, SARS‐CoV‐2) require laboratory confirmation of infection. During the current COVID‐19 pandemic, social distancing guidelines require alternative data collection in order to protect both research staff and participants. Home‐collected respiratory specimens are less resource‐intensive, can be collected earlier after symptom onset, and provide a low‐contact means of data collection. A prospective, multi‐year, community‐based cohort study is an ideal setting to examine the utility of home‐collected specimens for identification of influenza. We describe the feasibility and reliability of home‐collected specimens for the detection of influenza. We collected data and specimens between October 2014 and June 2017 from the Household Influenza Vaccine Evaluation (HIVE) Study. Cohort participants were asked to collect a nasal swab at home upon onset of acute respiratory illness. Research staff also collected nose and throat swab specimens in the study clinic within 7 days of onset. We estimated agreement using Cohen's kappa and calculated sensitivity and specificity of home‐collected compared to staff‐collected specimens. We tested 336 paired staff‐ and home‐collected respiratory specimens for influenza by RT‐PCR; 150 staff‐collected specimens were positive for influenza A/H3N2, 23 for influenza A/H1N1, 14 for influenza B/Victoria, and 31 for influenza B/Yamagata. We found moderate agreement between collection methods for influenza A/H3N2 (0.70) and B/Yamagata (0.69) and high agreement for influenza A/H1N1 (0.87) and B/Victoria (0.86). Sensitivity ranged from 78% to 86% for all influenza types and subtypes. Specificity was high for influenza A/H1N1 and both influenza B lineages with a range from 96% to 100%, and slightly lower for A/H3N2 infections (88%). Collection of nasal swab specimens at home is both feasible and reliable for identification of influenza virus infections.
登录
查看更多内容
影响因子：
11.8
作者：
Flannery, Brendan;Chung, Jessie R.;Barnes, John
通讯作者：
Barnes, John
影响因子：
3.9
作者：
Vargas, Celibell Y.;Wang, Liqun;Stockwell, Melissa S.
通讯作者：
Stockwell, Melissa S.
影响因子：
9.4
作者：
Shu, Bo;Wu, Kai-Hui;Lindstrom, Stephen
通讯作者：
Lindstrom, Stephen
影响因子：
3.7
作者：
Akmatov, Manas K.;Gatzemeier, Anja;Pessler, Frank
通讯作者：
Pessler, Frank
影响因子：
12.7
作者：
Granados, Andrea;Quach, Susan;Kwong, Jeffrey C.
通讯作者：
Kwong, Jeffrey C.