Genome-wide analysis of DNA G-quadruplex motifs across 37 species provides insights into G4 evolution.
Genome-wide analysis of DNA G-quadruplex motifs across 37 species provides insights into G4 evolution.
复制标题
对 37 个物种的 DNA G 四链体基序进行全基因组分析，为 G4 进化提供见解
DOI：
10.1038/s42003-020-01643-4
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发表时间：
2021-01-22
影响因子：
5.9
通讯作者：
Feng Q
中科院分区：
文献类型：
--
作者：
Wu F;Niu K;Cui Y;Li C;Lyu M;Ren Y;Chen Y;Deng H;Huang L;Zheng S;Liu L;Wang J;Song Q;Xiang H;Feng Q
G-四链体 (G4) 结构已在许多生物体的基因组中被预测，并被证明在多种细胞活动中发挥调节作用。然而，关于G4的进化历史和分布特征的信息却很少。在这里，研究了 37 个具有进化代表性的物种的潜在 G4 的全基因组特征。在进化过程中，G4 的数量、长度和密度普遍增加。七个物种的免疫荧光证实了 G4 的存在和进化模式。 G4 倾向于聚集在染色体中，并且在遗传区域中富集。短环 G4 在大多数物种中都是保守的，而环长度多样性也存在，特别是在哺乳动物中。转录因子中似乎越来越丰富的G4携带基因和直系同源基因的比例逐渐增加。检测G4s与DNA甲基化位点之间的拮抗关系。这些发现表明，生物体可能已经进化将G4发展成一种新型的可逆且复杂的转录调控机制，有利于高等生物体的多种生理活动。
G-quadruplex (G4) structures have been predicted in the genomes of many organisms and proven to play regulatory roles in diverse cellular activities. However, there is little information on the evolutionary history and distribution characteristics of G4s. Here, whole-genome characteristics of potential G4s were studied in 37 evolutionarily representative species. During evolution, the number, length, and density of G4s generally increased. Immunofluorescence in seven species confirmed G4s’ presence and evolutionary pattern. G4s tended to cluster in chromosomes and were enriched in genetic regions. Short-loop G4s were conserved in most species, while loop-length diversity also existed, especially in mammals. The proportion of G4-bearing genes and orthologue genes, which appeared to be increasingly enriched in transcription factors, gradually increased. The antagonistic relationship between G4s and DNA methylation sites was detected. These findings imply that organisms may have evolutionarily developed G4 into a novel reversible and elaborate transcriptional regulatory mechanism benefiting multiple physiological activities of higher organisms.
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影响因子：
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