Embryonic gene expression altered by maternal exposure to air pollution in rats
Embryonic gene expression altered by maternal exposure to air pollution in rats
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母亲暴露于空气污染导致大鼠胚胎基因表达改变
DOI：
10.1007/s11356-020-09413-9
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发表时间：
2020-06
影响因子：
5.8
通讯作者：
William K. K. Wu
中科院分区：
文献类型：
--
作者：
Zheng Li;Jianqing Ma;Youxi Lin;Jianxiong Shen;Zhanyong Wu;Matthew T. V. Chan;William K. K. Wu
众所周知，暴露于空气污染会增加心血管、肺部和代谢疾病的风险。越来越多的证据表明，怀孕期间暴露于空气污染会对早期胚胎发育和儿童健康产生负面影响。我们进行了RNA测序，以确定在空气污染暴露的大鼠胚胎失调的mRNA。基因本体论（GO）和京都基因和基因组百科全书（KEGG）途径分析用于分析去调节的mRNA的潜在细胞功能。我们的分析表明，在妊娠第9天，当子宫内暴露于> 200 μg/m3的细颗粒物时，总共有1678个mRNA差异表达，其中1098个mRNA下调，580个mRNA上调。基因本体论（GO）和京都基因和基因组百科全书（KEGG）途径分析显示，间隙连接，细胞粘附，轴突导向和神经营养因子信号传导途径的关键生物过程受到空气污染暴露。此外，mRNA调控网络的重建突出了Tbx 4，Bmp 4，Sox 10，Wnt 9 b，Bmp 7和Foxc 2的中心作用。这些数据表明，胚胎mRNA失调可能是空气污染相关先天性缺陷形成的基础。
Exposure to air pollution is known to increase the risks for cardiovascular, pulmonary and metabolic diseases. Growing evidences also indicated that air pollution exposure during pregnancy could negatively impact on early embryonic development and children’s health. We performed RNA sequencing to identify deregulated mRNAs in air pollution-exposed rat embryos. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyse the potential cellular functions of deregulated mRNAs. Our analysis indicated that a total of 1678 mRNAs were differentially expressed on gestation day 9 upon in utero exposure to fine particulate matter of > 200 μg/m3, among which 1098 mRNAs were downregulated and 580 mRNAs were upregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed gap junction, cell adhesion, axon guidance and the neurotrophin signalling pathway as key biological processes perturbed by air pollution exposure. Furthermore, reconstruction of the mRNA regulatory network highlighted the central roles of Tbx4, Bmp4, Sox10, Wnt9b, Bmp7 and Foxc2. These data suggested that embryonic mRNA deregulation may underlie the formation of air pollution-associated congenital defects.
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影响因子：
29.4
作者：
Ma, Stephanie;Bao, Jessie Y. J.;Guan, Xin-Yuan
通讯作者：
Guan, Xin-Yuan
影响因子：
11.8
作者：
Mariet, Anne-Sophie;Mauny, Frederic;Bernard, Nadine
通讯作者：
Bernard, Nadine
DOI：
--
发表时间：
2017-12
期刊：
Neuro endocrinology letters
影响因子：
--
作者：
R. Sram;M. Velemínský;M. Velemínský;J. Stejskalová
通讯作者：
R. Sram;M. Velemínský;M. Velemínský;J. Stejskalová
影响因子：
7.2
作者：
Engle, Elizabeth C.
通讯作者：
Engle, Elizabeth C.
影响因子：
1.9
作者：
Seungwoo Shin;D. Bae;Choon-Sik Park;Jong-Uk Lee;R. Kim;Sung Roul Kim;H. Chang;Jong Sook Park
通讯作者：
Seungwoo Shin;D. Bae;Choon-Sik Park;Jong-Uk Lee;R. Kim;Sung Roul Kim;H. Chang;Jong Sook Park