Complementary Fluorescence Emission and Second Harmonic Spectra Improve Bilayer Characterization
Complementary Fluorescence Emission and Second Harmonic Spectra Improve Bilayer Characterization
复制标题
互补荧光发射和二次谐波光谱改善双层表征
DOI：
10.1007/s10895-020-02487-1
复制
发表时间：
2020
影响因子：
2.7
通讯作者：
Peric, Miroslav
中科院分区：
文献类型：
--
作者：
Ranganathan, Radha;Burkin, Asher J.;Peric, Miroslav
补充研究Laurdan荧光发射和二次谐波（SH）光谱在非极性，质子和非质子极性溶剂和磷脂双层。SH的光谱计算使用熟悉的方法在电自旋共振光谱产生更好的分辨率。光谱拟合对数正态分布。SH光谱显示存在两个发射在质子极性和非极性溶剂和在双层凝胶和液相和非质子极性溶剂中的单线。在均相溶剂和双层中，各谱线的各半峰位置与峰位置呈相似的线性。这一共同的特征表明平面和非平面劳登构象分别在较长（红色）和较短（蓝色）波长的发射状态。之前在凝胶相中未检测到的较弱的432 nm蓝线在SH中是可区分的。温度轨迹的地区和峰位置的个别线带来了新的见解的性质的脂质包装和演变的域，表明不均匀的脂质包装，即使在凝胶相。蓝线识别为来自较紧密堆积区域中的Laurdan的发射，并且凝胶相中的占主导地位的445-448 nm红线在液相中移动到484 nm，作为来自根据温度和相处于不同松弛阶段的Laurdan-水耦合状态的发射。通过凝胶-液体转变，蓝线面积随温度的意外增加与共存的低曲率域和高曲率域以及Laurdan对极性较低的低曲率域的偏好一致。
Complementary investigation of Laurdan fluorescence emission and second harmonic (SH) spectra in nonpolar, protic and aprotic polar solvents and phospholipid bilayers was carried out. SH of spectra computed using methods familiar in electro spin resonance spectroscopy yielded better resolution. Spectra were fit to log-normal distributions. SH spectra showed presence of two emissions in protic polar and nonpolar solvents and in both bilayer gel and liquid phases and a single line in aprotic polar solvents. Each of the half maximal positions of each line in both homogenous solvents and bilayers, expresses similar linearity with peak position. This shared feature suggests planar and nonplanar Laurdan conformation respectively in the longer (red) and shorter (blue) wavelength emitting states. The weaker 432 nm blue line, not detected before in the gel phase, is distinguishable in the SH. Temperature trajectories of areas and peak positions of the individual lines bring new insight into the nature of lipid packing and evolution of domains, indicating inhomogeneous lipid packing even in the gel phase. The blue line identifies as emission from Laurdan in tighter packed regions and the dominant 445–448 nm red line in the gel phase shifting to 484 nm in the liquid phase as emission from Laurdan-water coupled states that are in varying stages of relaxation according to temperature and phase. Unexpected increase in the area of the blue line with temperature through the gel-liquid transition is consistent with coexisting low and high curvature domains and Laurdan’s preference for less polar low curvature domains.
登录
查看更多内容
LAURDAN 荧光检测的磷脂混合物中相特性的调节和动态*
影响因子：
3.3
作者：
T. Parasassi;G. Ravagnan;R. Rusch;E. Gratton
通讯作者：
E. Gratton
完全非极性系统中温度依赖性超快溶剂化动力学
DOI：
--
发表时间：
1993
期刊：
影响因子：
--
作者：
J. Fourkas;M. Berg
通讯作者：
M. Berg
劳丹分子在吸收光谱和荧光光谱中的共形跃迁
DOI：
--
发表时间：
2011
期刊：
影响因子：
--
作者：
Yu. P. Morozova;O. M. Zharkova;T. Y. Balakina;V. Y. Artyukhov;B. V. Korolev
通讯作者：
B. V. Korolev
影响因子：
2.9
作者：
Bacalum, Mihaela;Zorila, Bogdan;Radu, Mihai
通讯作者：
Radu, Mihai
影响因子：
3.4
作者：
Harris, FM;Best, KB;Bell, JD
通讯作者：
Bell, JD