MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE *
MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE *
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LAURDAN 荧光检测的磷脂混合物中相特性的调节和动态*
DOI:
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发表时间:
1993
影响因子:
3.3
通讯作者:
E. Gratton
中科院分区:
文献类型:
--
作者:
T. Parasassi;G. Ravagnan;R. Rusch;E. Gratton
Steady‐state and dynamic fluorescence properties of 6‐lauroyl‐2‐dimethylaminonaphthalene (Laurdan) have been used to ascertain the coexistence of separate phase domains and their dynamic properties in phospholipid vesicles composed of different mole ratios of dilauroyl‐ and dipalmitoyl‐phosphatidylcholine (DLPC and DPPC, respectively). The recently introduced generalized polarization together with time‐resolved emission spectra have been utilized for detecting changes. The results indicate the coexistence of phospholipid phase domains in vesicle compositions in the range between 30 mol% and 70 mol% DPPC in DLPC. Below and above these concentrations a homogeneous phase is observed, with averaged properties. In the case of coexisting phase domains, the properties of each individual phase are largely influenced by the presence of the other phase. Implications on fluctuations between the coexisting phases and on the size and shape of domains are discussed.
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影响因子:
3.4
作者:
Vauhkonen,M;Sassaroli,M;Somerharju,P;Eisinger,J
通讯作者:
Eisinger,J
影响因子:
2.9
作者:
Gasser,KW;Goldsmith,A;Hopfer,U
通讯作者:
Hopfer,U
影响因子:
6.1
作者:
Tomita-Yamaguchi,M;Rubio,C;Santoro,TJ
通讯作者:
Santoro,TJ
影响因子:
3.4
作者:
Sassaroli,M;Vauhkonen,M;Perry,D;Eisinger,J
通讯作者:
Eisinger,J