MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE *

MODULATION AND DYNAMICS OF PHASE PROPERTIES IN PHOSPHOLIPID MIXTURES DETECTED BY LAURDAN FLUORESCENCE *
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LAURDAN 荧光检测的磷脂混合物中相特性的调节和动态*

DOI:
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发表时间:
1993
影响因子:
3.3
通讯作者:
E. Gratton
E. Gratton
中科院分区:
生物学3区
文献类型:
--
作者:
T. Parasassi;G. Ravagnan;R. Rusch;E. Gratton

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用6-月桂酰基-2-二甲氨基萘(Laurdan)的稳态和动态荧光性质研究了不同摩尔比的二月桂酰基和二棕榈酰基磷脂酰胆碱(DLPC和DPPC)组成的磷脂微囊中不同相区的共存及其动态性质。最近引入的广义极化和时间分辨发射光谱被用于检测变化。结果表明,在DLPC中,DPPC的囊泡组成中存在磷脂相结构域,范围为30~70mol%DPPC。在这些浓度以下和以上,观察到具有平均性质的均一相。在共存相区的情况下,每个单独相的性质很大程度上受另一个相的存在的影响。讨论了共存相之间的波动以及磁区的大小和形状的影响。
Steady‐state and dynamic fluorescence properties of 6‐lauroyl‐2‐dimethylaminonaphthalene (Laurdan) have been used to ascertain the coexistence of separate phase domains and their dynamic properties in phospholipid vesicles composed of different mole ratios of dilauroyl‐ and dipalmitoyl‐phosphatidylcholine (DLPC and DPPC, respectively). The recently introduced generalized polarization together with time‐resolved emission spectra have been utilized for detecting changes. The results indicate the coexistence of phospholipid phase domains in vesicle compositions in the range between 30 mol% and 70 mol% DPPC in DLPC. Below and above these concentrations a homogeneous phase is observed, with averaged properties. In the case of coexisting phase domains, the properties of each individual phase are largely influenced by the presence of the other phase. Implications on fluctuations between the coexisting phases and on the size and shape of domains are discussed.
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