Altered properties and structures of root exudate polysaccharides in a root hairless mutant of barley.
Altered properties and structures of root exudate polysaccharides in a root hairless mutant of barley.
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DOI：
10.1093/plphys/kiac341
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发表时间：
2022-09-28
期刊：
影响因子：
7.4
通讯作者：
Knox, Paul
中科院分区：
文献类型：
--
作者：
Galloway, Andrew F.;Akhtar, Jumana;Burak, Emma;Marcus, Susan E.;Field, Katie J.;Dodd, Ian C.;Knox, Paul
根系分泌物和附着土壤的根鞘是根系生长的重要特征。为了阐明根鞘形成的相关因素，以野生型大麦（Hordeum vulgare L.）CV.采用生理生化和免疫化学相结合的方法，研究了根无毛突变体大麦（brb）和根无毛突变体大麦（Pallas）的遗传特性。当在土壤中生长时，WT大麦根每单位根长结合的土壤是brb的2.5倍。高分子量（HMW）多糖分泌物的brb根有较低的土壤结合能力比WT根分泌物。HMW多糖渗出物的碳水化合物和聚糖单克隆抗体分析表明聚糖谱不同。相对于野生型植物，根分泌物的brb减少信号阿拉伯半乳聚糖蛋白（AGP），伸展蛋白，和heteroxylan表位。相比之下，2周龄brb植物的根分泌物含有相对于WT多25倍的可检测的木葡聚糖表位。根系免疫印迹证实，相对于WT，从brb根尖和根轴释放的木葡聚糖表位水平更高。用阴离子交换色谱法进行的表位检测表明，在brb渗出物中木葡聚糖的检测增加是由于中性聚合物丰度的增加。相反，BRB根分泌物含有减少量的酸性聚合物，具有土壤结合特性，含有木葡聚糖表位和糖蛋白和杂木聚糖表位相对于WT。因此，我们建议，除了物理结构的土壤颗粒，根毛促进根鞘的形成，通过释放土壤结合多糖复合物。根无毛突变大麦，相对于野生型的根分泌物，具有改变的多糖表位模式和较少量的酸性土壤结合多糖复合物。
Root exudates and rhizosheaths of attached soil are important features of growing roots. To elucidate factors involved in rhizosheath formation, wild-type (WT) barley (Hordeum vulgare L. cv. Pallas) and a root hairless mutant, bald root barley (brb), were investigated with a combination of physiological, biochemical, and immunochemical assays. When grown in soil, WT barley roots bound ∼5-fold more soil than brb per unit root length. High molecular weight (HMW) polysaccharide exudates of brb roots had less soil-binding capacity than those of WT root exudates. Carbohydrate and glycan monoclonal antibody analyses of HMW polysaccharide exudates indicated differing glycan profiles. Relative to WT plants, root exudates of brb had reduced signals for arabinogalactan-protein (AGP), extensin, and heteroxylan epitopes. In contrast, the root exudate of 2-week-old brb plants contained ∼25-fold more detectable xyloglucan epitope relative to WT. Root system immunoprints confirmed the higher levels of release of the xyloglucan epitope from brb root apices and root axes relative to WT. Epitope detection with anion-exchange chromatography indicated that the increased detection of xyloglucan in brb exudates was due to enhanced abundance of a neutral polymer. Conversely, brb root exudates contained decreased amounts of an acidic polymer, with soil-binding properties, containing the xyloglucan epitope and glycoprotein and heteroxylan epitopes relative to WT. We, therefore, propose that, in addition to physically structuring soil particles, root hairs facilitate rhizosheath formation by releasing a soil-binding polysaccharide complex. The root exudate of a root hairless mutant of barley, relative to wild type, has an altered pattern of polysaccharide epitopes and lesser amounts of an acidic soil-binding polysaccharide complex.
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DOI：
10.1111/nph.14705
发表时间：
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期刊：
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影响因子：
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影响因子：
6.9
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