Arabinogalactan proteins are involved in root hair development in barley.
Arabinogalactan proteins are involved in root hair development in barley.
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DOI：
10.1093/jxb/eru475
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发表时间：
2015-03
影响因子：
6.9
通讯作者：
Melzer M
中科院分区：
文献类型：
--
作者：
Marzec M;Szarejko I;Melzer M
LM2、LM14和MAC207抗体识别的阿拉伯半乳聚糖蛋白（Arabinogalactan protein, AGPs）在大麦根生毛基因型的根真皮中有不同的沉积，而在根无毛突变体的根真皮细胞中分布均匀。阿拉伯半乳聚糖蛋白（AGPs）参与一系列植物过程，包括细胞分化和扩增。本研究以大麦根毛突变体及其野生型亲本品种为模型，揭示了AGPs在根毛发育中的作用。不同浓度的βGlcY（一种能与所有种类的AGPs结合的试剂）对根的处理抑制或完全抑制了所有品种根毛的发育。三组AGP（被单克隆抗体LM2、LM14和MAC207识别）不同地定位于根生毛植物的毛成细胞和非毛成细胞中。相关的表位存在于野生型毛原细胞细胞壁和细胞质中，而在野生型无毛细胞和无毛突变体的所有表皮细胞中，它们仅存在于细胞质中。在所有品种中，在毛胚发育早期，LM2、LM14和MAC207的表达均较高。此外，在能够产生根毛的植物的原基和根毛管表面检测到LM2表位。主要结论是，LM2、LM14和MAC207识别的AGPs参与了大麦根表皮细胞的分化，从而提示大麦根毛发育需要这些AGPs。
Arabinogalactan proteins (AGPs) recognized by the LM2, LM14, and MAC207 antibodies are diversely deposited in rhizodermis of barley root hair-producing genotypes, but evenly deposited in rhizodermal cells of the root hairless mutant. The arabinogalactan proteins (AGPs) are involved in a range of plant processes, including cell differentiation and expansion. Here, barley root hair mutants and their wild-type parent cultivars were used, as a model system, to reveal the role of AGPs in root hair development. The treatment of roots with different concentrations of βGlcY (a reagent which binds to all classes of AGPs) inhibited or totally suppressed the development of root hairs in all of the cultivars. Three groups of AGP (recognized by the monoclonal antibodies LM2, LM14, and MAC207) were diversely localized in trichoblasts and atrichoblasts of root hair-producing plants. The relevant epitopes were present in wild-type trichoblast cell walls and cytoplasm, whereas in wild-type atrichoblasts and in all epidermal cells of a root hairless mutant, they were only present in the cytoplasm. In all of cultivars the higher expression of LM2, LM14, and MAC207 was observed in trichoblasts at an early stage of development. Additionally, the LM2 epitope was detected on the surface of primordia and root hair tubes in plants able to generate root hairs. The major conclusion was that the AGPs recognized by LM2, LM14, and MAC207 are involved in the differentiation of barley root epidermal cells, thereby implying a requirement for these AGPs for root hair development in barley.
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影响因子：
6.4
作者：
Lu, H;Chen, M;Showalter, AM
通讯作者：
Showalter, AM
影响因子：
4.3
作者：
Kwasniewski, Miroslaw;Janiak, Agnieszka;Szarejko, Iwona
通讯作者：
Szarejko, Iwona
影响因子：
7.4
作者：
Dardelle, Flavien;Lehner, Arnaud;Mollet, Jean-Claude
通讯作者：
Mollet, Jean-Claude
大麦根表皮细胞中增加的互渗透性与根毛发育的缺陷相关。
DOI：
10.1111/plb.12066
发表时间：
2014-03
期刊：
Plant biology (Stuttgart, Germany)
影响因子：
--
作者：
Marzec M;Muszynska A;Melzer M;Sas-Nowosielska H;Kurczynska EU
通讯作者：
Kurczynska EU
影响因子：
4.3
作者：
Casero, PJ;Casimiro, I;Knox, JP
通讯作者：
Knox, JP