Characteristics and significance of T lymphocyte subsets in peripheral blood of osteosarcoma mice.
Characteristics and significance of T lymphocyte subsets in peripheral blood of osteosarcoma mice.
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DOI：
10.21037/tcr-22-264
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发表时间：
2022-06
影响因子：
0.9
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中科院分区：
文献类型：
--
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骨肉瘤（osteosarcoma，OS）是最常见的恶性骨肿瘤，常伴有肺转移.肿瘤转移后的生存率很低。包括天然免疫细胞的抗肿瘤免疫活性在内的治疗被发现对OS肿瘤有利。然而，作为早期肿瘤检测的主要手段，对OS患者外周血T细胞的研究仍然很少。将OS细胞植入C3 H/HeN小鼠股骨骨髓腔内建立OS模型。于造模后第14、21天采用流式细胞仪检测小鼠外周血T淋巴细胞亚群比例。与假手术组比较，术后第14天，γδT细胞和CD 4+调节性T细胞（Treg）的比例显著升高，其他各亚群无明显差异。术后第21天，CD 3 + T细胞、γδ T细胞、CD 4 + T细胞亚群及T细胞亚群/Th 17比值均明显高于假手术组。同时，CD 4 + T细胞和CD 8 + T细胞均较低，提示外周免疫抑制的发生。比较肿瘤组造模后第14天和第21天T淋巴细胞亚群，发现造模后第21天的CD 3 + T细胞和CD 4 + T淋巴细胞明显高于第14天，而CD 4 + T细胞和CD 8 + T细胞明显下降，表明OS小鼠外周血T淋巴细胞的变化与肿瘤的发生有关。股骨OS的形成和发展伴随着外周血T淋巴细胞亚群的紊乱和外周免疫抑制。
Osteosarcoma (OS) is the most common malignant bone tumor, which often has lung metastasis. The survival rate after tumor metastasis is very low. Treatments including the antitumor immunocompetence of innate immune cells are found favorable for OS tumor. However, as a major means of early tumor detection, there are still few studies on T cells in peripheral blood of OS patients. OS cells were implanted into the femoral bone marrow cavity of C3H/HeN mice to construct OS model. The proportion of T lymphocyte subsets in peripheral blood of mice was detected by flow cytometry on the 14th and 21st days after modeling. Compared with sham operation group, on the 14th day after operation, the proportion of γδT cells and CD4+ regulatory T cells (Tregs) showed significantly higher level while other subsets didn’t have obvious difference. On the 21st day after operation, the proportion of CD3+ T cells, γδ T cells, CD4+ Tregs and Tregs/Th17 ratio were obviously higher than that of sham operation group. Meanwhile, while CD4+ T cells and CD8+ T cells were lower, suggesting the happen of peripheral immunosuppression. Comparing the T lymphocytes subsets on the 14th and 21st day after modeling in the tumor group, it was found that CD3+ T cells and CD4+ Tregs on the 21st day after modeling were significantly higher than those on the 14th day while CD4+ T cells, CD8+ T cells decreased significantly, indicating the changes of T lymphocytes in peripheral blood of OS mice are related to tumor development. The formation and development of femoral OS is accompanied by the disorder of peripheral blood T lymphocyte subsets and peripheral immunosuppression.
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