Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes.
Uninephrectomized High-Fat-Fed Nicotinamide-Streptozotocin-Induced Diabetic Rats: A Model for the Investigation of Diabetic Nephropathy in Type 2 Diabetes.
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DOI：
10.1155/2016/8317850
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发表时间：
2016
影响因子：
4.3
通讯作者：
Grineva EN
中科院分区：
文献类型：
--
作者：
Bayrasheva VK;Babenko AY;Dobronravov VA;Dmitriev YV;Chefu SG;Pchelin IY;Ivanova AN;Bairamov AA;Alexeyeva NP;Shatalov IS;Grineva EN
通过使用低剂量的链脲佐菌素 (LD-STZ) 以及预先给予烟酰胺 (NA) 的高剂量 STZ，可以在饮食性肥胖大鼠中重现 2 型糖尿病 (DM2)。然而，STZ 可能引起肾小管毒性。目的。开发 NA-STZ 诱导的 DM2 中的 DN 大鼠模型，并将其与 LD-STZ 模型进行比较，以便选择最相关的方法来重现肾小球和肾小管形态功能糖尿病变化。从单肾切除术后 3 周开始，成年雄性 Wistar 大鼠饲喂 5 周高脂饮食，然后腹腔注射 LD-STZ (40 mg/kg) 或 NA (230 mg/kg)，随后注射 STZ (65 mg/kg)。对照未切除肾的注射媒介物的大鼠接受正常饮食。在第10周、第20周和第30周（研究结束）时，评估了代谢参数、肌酐清除率、白蛋白尿和尿管损伤标志物（NGAL、KIM-1），以及第20周和第30周时的肾脏超微结构和光镜变化。NA-STZ组表现出更高的代谢参数的再现性和稳定性。到第 10 周，NA-STZ 组的 NGAL 水平显着低于 LD-STZ 组。到第 30 周时，糖尿病组表现出 DN 的早期特征。然而，尽管 KIM-1 和 NGAL 水平较低，但 NA-STZ 组的形态功能变化似乎比 STZ 组更明显。我们提出了一种新的 DM2 糖尿病大鼠模型，其特征是与 LD-STZ 诱导的糖尿病相比，稳定的代谢紊乱、典型的肾脏病变和肾小管损伤标志物水平较低。
Type 2 diabetes (DM2) could be reproduced in rats with alimentary obesity by using low doses of streptozotocin (LD-STZ) as well as STZ in high doses with preliminary nicotinamide (NA) administration. However, STZ could induce tubulotoxicity. Aim. To develop rat model of DN in NA-STZ-induced DM2 and compare it with LD-STZ-model in order to choose the most relevant approach for reproducing renal glomerular and tubular morphofunctional diabetic changes. Starting at 3 weeks after uninephrectomy, adult male Wistar rats were fed five-week high-fat diet and then received intraperitoneally either LD-STZ (40 mg/kg) or NA (230 mg/kg) followed by STZ (65 mg/kg). Control uninephrectomized vehicle-injected rats received normal chow. At weeks 10, 20, and 30 (the end of the study), metabolic parameters, creatinine clearance, albuminuria, and urinary tubular injury markers (NGAL, KIM-1) were evaluated as well as renal ultrastructural and light microscopic changes at weeks 20 and 30. NA-STZ-group showed higher reproducibility and stability of metabolic parameters. By week 10, in NA-STZ-group NGAL level was significantly lower compared to LD-STZ-group. By week 30, diabetic groups showed early features of DN. However, morphofunctional changes in NA-STZ-group appeared to be more pronounced than those in STZ-group despite lower levels of KIM-1 and NGAL. We proposed a new rat model of DM2 with DN characterized by stable metabolic disorders, typical renal lesions, and lower levels of tubular injury markers as compared to LD-STZ-induced diabetes.
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影响因子：
3.7
作者：
Nakagawa S;Nishihara K;Miyata H;Shinke H;Tomita E;Kajiwara M;Matsubara T;Iehara N;Igarashi Y;Yamada H;Fukatsu A;Yanagita M;Matsubara K;Masuda S
通讯作者：
Masuda S
影响因子：
2.2
作者：
Khan E;Batuman V;Lertora JJ
通讯作者：
Lertora JJ
DOI：
10.1111/j.1751-7176.2011.00434.x
发表时间：
2011-04
期刊：
Journal of clinical hypertension (Greenwich, Conn.)
影响因子：
--
作者：
Long AN;Dagogo-Jack S
通讯作者：
Dagogo-Jack S
DOI：
10.1155/2012/140103
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Liu IM;Tzeng TF;Liou SS;Chang CJ
通讯作者：
Chang CJ
影响因子：
2.8
作者：
de Carvalho, Jose Antonio M.;Tatsch, Etiane;Moresco, Rafael N.
通讯作者：
Moresco, Rafael N.