Simplified stereological evaluation of renal morphology after unilateral ureteral obstruction.
Simplified stereological evaluation of renal morphology after unilateral ureteral obstruction.
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单侧输尿管梗阻后肾脏形态的简化体视学评估。
DOI：
10.7150/ijms.5182
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发表时间：
2013
影响因子：
3.6
通讯作者：
Qin YH
中科院分区：
文献类型：
--
作者：
Hu P;Zhao XQ;Wang J;Cai J;Hu B;Lu L;Xuan Q;Qin YH
目的和方法：本研究建立了一个简单的体视学方法，以获得定量信息的二维或三维结构的基础上观察肾脏切片的单侧输尿管梗阻（UUO）模型。结果如下：与假手术大鼠（SOR）相比，梗阻肾在结扎后1 ~ 3个月肾小管间质面积（TA）和TA/矩形视野面积（RA）逐渐增加，但差异显著。相反，肾小球面积（GA）和肾小球体积（GV）随时间进行性减少，但在3周至3个月的梗阻肾与SOR假肾相比，显着。UUO引起对侧肾TA和TA/RA进行性下降。结扎后1、2、3个月TA较结扎前明显降低，而TA/RA仅在结扎后3个月时较结扎前明显降低。而对侧肾GA和GV则呈时间依赖性增强，其中GA在结扎后3个月才达到显著性差异，而GV在结扎后1 ~ 3个月才达到显著性差异。结论：我们的结果证实了梗阻性肾病的两个典型特征，即梗阻肾的间质间隙增宽和肾小球萎缩，以及对侧肾的代偿性生长。
Objective and methods: This study established a simple stereological method to obtain quantitative information about two- or three-dimensional structures based on observations from kidney sections in the unilateral ureteral obstruction(UUO) model. Results: Tubulointerstitial area(TA) and TA/the area of a rectangular field(RA) were raised gradually, but significantly, in the obstructed kidney from 1 to 3months post-ligation in comparison to the sham kidney of sham-operated rats(SOR). On the contrary, glomerular area(GA) and glomerular volume(GV) were decreased progressively over time, but significantly, in the obstructed kidney from 3weeks to 3months post-ligation compared to the sham kidney of SOR. UUO caused a progressive decline of TA and TA/RA in the contralateral kidney. More specifically, there were significant decreases in TA at 1,2,3months post-ligation, while in TA/RA only at 3months post-ligation in comparison to the right kidney of SOR. In contrast, GA and GV enhanced in a time-dependent manner in the contralateral kidney, in which the difference in GA reached significance only at 3months post-ligation, whereas the difference in GV reached significance from 1 to 3months post-ligation when comparing with the right kidney of SOR. Conclusions: Our results confirmed two typical features of obstructive nephropathy, including widen interstitial space and glomerular atrophy in the obstructed kidney, and compensatory growth of the contralateral kidney.
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DOI：
10.1152/ajprenal.1999.277.1.f41
发表时间：
1999-07-01
影响因子：
4.2
作者：
Chevalier, RL;Thornhill, BA;Wolstenholme, JT
通讯作者：
Wolstenholme, JT
尿C型利钠肽排泄增加可能是肾小管间质纤维化的早期标志
影响因子：
3
作者：
Hu, Peng;Wang, Jing;Qin, Yuan Han
通讯作者：
Qin, Yuan Han
过表达的 C 型利尿钠肽可作为对抗单侧输尿管梗阻大鼠细胞外基质重塑的早期代偿反应
影响因子：
2.8
作者：
Hu, Peng;Wang, Jing;Qin, Yuan Han
通讯作者：
Qin, Yuan Han
影响因子：
19.6
作者：
Hammad, FT;Wheatley, AM;Davis, G
通讯作者：
Davis, G
影响因子：
3
作者：
Hodgkins, Kavita S.;Schnaper, H. William
通讯作者：
Schnaper, H. William