Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups.
Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups.
复制标题
10-23 DNAzyme催化核心中两个胸腺嘧啶残基的研究：对其5位取代官能团催化作用的影响
DOI：
10.3390/molecules22071011
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发表时间：
2017-06-22
期刊：
影响因子：
--
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Li P;Du S;Li Y;He J
在10-23DNAzyme的15聚体催化核心中，每个残基对催化构象的贡献不同。在这里，临界保守的T4和最不保守的T8在其5位上被羟基、咪唑基和具有氢键能力的氨基修饰。这些外部官能团在催化核内部诱导了新的相互作用，对10-23DNAzyme的催化活性产生了正负两种影响，不同的键可以用来调节官能团的作用。在碱基水平上可以识别T4和T8的保守性，但在功能基团水平上T4不是完全保守的。可以对它们的5-甲基进行修饰，以获得更好的DNA酶性能。
In the 15-mer catalytic core of 10-23 DNAzyme, each residue contributes to the catalytic conformation differently. Here, the critically conserved T4 and the least conserved T8 were modified on their 5-position with hydroxyl, imidazolyl, and amino groups with a hydrogen-bonding ability. These external functional groups induced new interactions within the catalytic core, resulting in both negative and positive effects on the catalytic activity of 10-23 DNAzyme, and the different linkages could be used to modulate the effect of the functional groups. The conservation of T4 and T8 could be recognized at the level of the nucleobase, but at the level of the functional group, T4 is not completely conserved. Their 5-methyl groups could be modified for a better performance in terms of the DNAzyme.
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DOI：
10.1073/pnas.94.9.4262
发表时间：
1997-04-29
影响因子：
11.1
作者：
Santoro, SW;Joyce, GF
通讯作者：
Joyce, GF
DOI：
10.1007/s00018-008-8029-y
发表时间：
2008-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Baum DA;Silverman SK
通讯作者：
Silverman SK
影响因子：
4.5
作者：
Breaker, RR;Emilsson, GM;Sudarsan, N
通讯作者：
Sudarsan, N
影响因子：
5.2
作者：
Lusic, Hrvoje;Young, Douglas D.;Deiters, Alexander
通讯作者：
Deiters, Alexander
影响因子：
5.4
作者：
Nawrot, Barbara;Widera, Kinga;Stec, Wojciech J.
通讯作者：
Stec, Wojciech J.