The role of PhoP/PhoQ two component system in regulating stress adaptation in Cronobacter sakazakii.
The role of PhoP/PhoQ two component system in regulating stress adaptation in Cronobacter sakazakii.
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PhoP/PhoQ 双组分系统在调节坂崎克罗诺杆菌应激适应中的作用
DOI：
10.1016/j.fm.2021.103851
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发表时间：
2021-06
影响因子：
5.3
通讯作者：
Xia Xiaodong
中科院分区：
文献类型：
--
作者：
Ma Yan;Zhang Yingying;Chen Ke;Zhang Lingzhu;Zhang Yibei;Wang Xin;Xia Xiaodong
坂崎克罗诺杆菌是一种机会性食源性细菌病原体，对多种应激条件表现出抵抗力。 PhoP/PhoQ二元系统是多种细菌应激反应和毒力的关键调节机制，但其在C.坂崎还没有被彻底研究过。在这项研究中，我们在C中发现了PhoP/PhoQ系统。 sakazakiiATCC BAA-894 在低 Mg2+、酸性 pH 和存在多粘菌素 B 的条件下增强细菌生长。此外，与 WT 菌株相比，ΔphoPQ 菌株在暴露于热、高渗透压、氧化盐或胆盐后显着降低存活率。此外，RNA-seq分析表明，ΔphoPQstrain中有1029个基因上调，979个基因下调。细菌分泌系统、鞭毛组装、β-内酰胺抗性和双组分系统途径显着下调，而ABC转运蛋白和不同环境下的微生物代谢途径则上调。 qRT-PCR分析进一步证实与应激耐受相关的12个基因受到PhoP/PhoQ系统的正向调控。因此，这些发现表明PhoP/PhoQ系统是C.的重要调控机制。坂崎藤能够抵抗各种环境压力。
Cronobacter sakazakiiis an opportunistic foodborne bacterial pathogen that shows resistance to multiple stress conditions. The PhoP/PhoQ two component system is a key regulatory mechanism of stress response and virulence in various bacteria, but its role inC. sakazakiihas not been thoroughly studied. In this study, we found the PhoP/PhoQ system inC. sakazakiiATCC BAA-894 enhanced bacterial growth in conditions with low Mg2+, acid pH, and the presence of polymyxin B. Moreover, the ΔphoPQstrain significantly reduced survival following exposure to heat, high osmotic pressure, oxidative or bile salts compared with WT strain. Furthermore, the RNA-seq analysis indicated that 1029 genes were upregulated and 979 genes were downregulated in ΔphoPQstrain. The bacterial secretion system, flagella assembly, beta-Lactam resistance and two-component system pathways were significantly downregulated, while the ABC transporters and microbial metabolism in diverse environments pathways were upregulated. qRT-PCR analysis further confirmed that twelve genes associated with stress tolerance were positively regulated by the PhoP/PhoQ system. Therefore, these findings suggest that the PhoP/PhoQ system is an important regulatory mechanism forC. sakazakiito resist various environmental stress.
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