Pre-existing immunity to pathogenic Listeria monocytogenes does not prevent induction of immune responses to feline immunodeficiency virus by a novel recombinant Listeria monocytogenes vaccine.
Pre-existing immunity to pathogenic Listeria monocytogenes does not prevent induction of immune responses to feline immunodeficiency virus by a novel recombinant Listeria monocytogenes vaccine.
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对致病性单核细胞增生李斯特菌的预先存在的免疫力不会阻止新型重组单核细胞增生李斯特菌疫苗诱导对猫免疫缺陷病毒的免疫反应。
DOI：
10.1016/j.vaccine.2004.09.033
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Dean,GreggA
中科院分区：
文献类型：
--
作者：
Stevens,Rosemary;Lavoy,Alora;Nordone,Shila;Burkhard,Maryjo;Dean,GreggA
单核细胞增多性李斯特菌是一种很有吸引力的抗HIV生物疫苗载体，因为它能诱导强烈的细胞免疫应答，可以通过粘膜途径传递，易于操纵来表达病毒抗原，而且生产简单且成本低廉。概念验证研究已经在小鼠中使用表达重组单核细胞增多性乳杆菌的HIV Gag进行。在这里，我们报告了将在HIV的FIV/CAT模型中评估的重组单核细胞增多性乳杆菌的开发和验证。用一种简化的方法引入单个和多个FIV Gag基因，我们证明了含有完整Gag的重组单核细胞增多性乳杆菌以可溶分泌的形式表达全长Gag多蛋白。将在革兰氏阳性菌中复制的含有FIV SU(Gp100)和TM胞外区(Gp40)的DNA疫苗质粒(pND14-LC-env)导入LM-Gag，构建了LM-Gag/pND14-LC-env。Lm-Gag/pND14-LC-env体外感染靶细胞后，在可溶性细胞裂解物中同时检测到FIV Gag和Env蛋白。在口服和/或皮下感染野生型单核细胞增多性李斯特菌的猫中，确定既往暴露于单核细胞增多性李斯特菌是否影响LM-Gag/pND14-LC-env的免疫原性。单次口服LM-Gag/pND14-LC-env后，已有抗L.单核细胞增多性免疫反应在阴道分泌物、唾液和粪便中产生抗FIV Gag IgA效价。同样，FIVGag和Env特异性的干扰素-γELISPOT反应在脾和淋巴中都可以测量到，但在单次皮下注射野生型单核细胞增多性李斯特菌的猫中，其频率在统计上高于皮下和口服暴露的猫。FIV/CAT模型将提供一个有用的攻击系统，以确定重组单核细胞增多性李斯特菌在通过常见的HIV传播途径攻击后，是否能够在其自然宿主中保护慢病毒。
Listeria monocytogenes is an attractive biologic vaccine vector against HIV because it induces a strong cell mediated immune response, can be delivered by mucosal routes, can be readily manipulated to express viral antigens, and is easy and inexpensive to produce. Proof of concept studies have been performed using HIV Gag expressing recombinant L. monocytogenes in the mouse. Here we report the development and validation of recombinant L. monocytogenes to be evaluated in the FIV/cat model of HIV. Using a simplified approach to introduce individual and polyprotein FIV gag genes, we show that recombinant L. monocytogenes containing the entire gag expresses the full-length Gag polyprotein in a soluble secreted form. A DNA vaccine plasmid (pND14-Lc-env) that replicates in Gram positive bacteria and contains the FIV SU (gp100) and the ectodomain of TM (gp40) in a eukaryotic expression cassette was transfected into LM-gag to create LM-gag/pND14-Lc-env. After infection of target cells with LM-gag/pND14-Lc-env in vitro, both FIV Gag and Env proteins were detected in soluble cell lysates. Whether previous exposure to L. monocytogenes affects the immunogenicity of LM-gag/pND14-Lc-env was determined in cats infected with wild-type L. monocytogenes orally and/or subcutaneously. After a single oral dose of LM-gag/pND14-Lc-env, cats with existing anti-L. monocytogenes immune responses developed anti-FIV Gag IgA titers in vaginal secretions, saliva, and feces. Similarly, FIV Gag and Env specific IFN-γ ELISPOT responses were measurable in spleen and lymph node but at a statistically higher frequency in cats exposed to a single subcutaneous dose of wild-type L. monocytogenes versus cats exposed both subcutaneously and orally. The FIV/cat model will provide a useful challenge system to determine whether recombinant L. monocytogenes can protect against a lentivirus in its natural host after challenge by the routes common to HIV transmission.
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对 MHC 缺陷型敲除小鼠的研究揭示了 MHC I 和 MHC II 依赖性 T 细胞反应对单核细胞增生李斯特菌感染的影响。
影响因子：
4.4
作者：
C. Ladel;I. Flesch;J. Arnoldi;S. Kaufmann
通讯作者：
S. Kaufmann
影响因子：
2.7
作者：
Geiser, M;Cèbe, R;Schmitz, R
通讯作者：
Schmitz, R
小鼠原发性和继发性李斯特菌感染中迟发型超敏反应、保护和肉芽肿形成中的 T 细胞亚群：Lyt-2 细胞在获得性免疫中的卓越作用
影响因子：
3.1
作者：
Martin E. A. Mielke;Stefan Ehlers;Helmut Hahn
通讯作者：
Helmut Hahn
DOI：
10.1073/pnas.92.9.3987
发表时间：
1995-04-25
影响因子：
11.1
作者：
SHEN, H;SLIFKA, MK;MILLER, JF
通讯作者：
MILLER, JF
FIV 作为艾滋病疫苗接种的典范
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
Calvin M. Johnson;B. Torres;H. Koyama;J. Yamamoto
通讯作者：
J. Yamamoto