Morphological and northern blot analysis of juxtaglomerular cells in experimental hydronephrotic mice
Morphological and northern blot analysis of juxtaglomerular cells in experimental hydronephrotic mice
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实验性肾积水小鼠肾小球旁细胞的形态学和 Northern blot 分析
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. Sugimura
中科院分区：
文献类型：
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作者：
Y. Kon;S. Takahashi;A. Fukamizu;K. Murakami;Y. Hashimoto;M. Sugimura
本研究的目的是利用免疫组织化学、组织平面测量和Northern blot技术来证明实验性肾积水的发育变化。输尿管结扎1个月后，免疫组织化学检测到大量肾素阳性细胞，即使在1:10 000的稀释下，也能在这个肾积水肾脏中检测到；然而，在非结扎侧很少有肾素阳性细胞。结扎6个月后，结扎和未结扎肾脏的肾素反应性无差异。在肾素阳性区的形态计量学分析中，结扎侧数值在2周时已经升高，在1个月时达到最高值，然后在实验结束时逐渐下降到与对照肾脏几乎相同的值。另一方面，单侧结扎后，未结扎侧肾脏的价值立即下降，随后增加，最终达到与对照肾脏几乎相同的价值。在Northern blot分析中，结扎后1个月结扎侧肾素mRNA的活性明显高于未结扎侧。然而，结扎侧和未结扎侧之间的差异在6个月时未得到证实，其价值几乎与未手术肾脏相同。
The purpose of this study is to demonstrate the developmental changes of the experimental hydronephrotic kidney using immunohistochemical, histoplanimetrical, and Northern blot techniques. At 1 month after ligation of the ureter, a large number of renin‐positive cells were detected immunohistochemically even at a dilution of 1:10,000 in this hydronephrotic kidney; however, there were few renin‐positive cells in the non‐ligated side. At 6 months after ligation, no difference in reactivity for renin between ligated and non‐ligated kidneys was demonstrated. In the morphometrical analysis of the renin‐positive region, the numerical value of the ligated side was already increased at 2 weeks, reached the highest value at 1 month, and then decreased gradually to almost the same value as the control kidney by the end of the experiment. On the other hand, the value of the non‐ligated side decreased immediately after the unilateral ligation, increased later, and finally reached almost the same value as the control kidney. In the Northern blot analysis, the activity of renin mRNA in the ligated side at 1 month after ligation was markedly higher than that in the non‐ligated side. However, the difference between the ligated and the non‐ligated sides was not demonstrated at 6 months and the value came to be almost the same as in the nonoperated kidney.
叙利亚仓鼠 DES 诱导肾肿瘤的免疫组织化学肾素研究。
DOI：
10.1002/aja.1001820406
发表时间：
1988
期刊：
The American journal of anatomy
影响因子：
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作者：
Dodge,AH;Brownfield,M;Reid,IA;Inagami,T
通讯作者：
Inagami,T