In vivo two-photon imaging of T cell motility in joint-draining lymph nodes in a mouse model of rheumatoid arthritis.
In vivo two-photon imaging of T cell motility in joint-draining lymph nodes in a mouse model of rheumatoid arthritis.
复制标题
DOI：
10.1016/j.cellimm.2012.08.003
复制
发表时间：
2012-07
影响因子：
4.3
通讯作者：
Mikecz K
中科院分区：
文献类型：
--
作者：
Kobezda T;Ghassemi-Nejad S;Glant TT;Mikecz K
最近对幼稚T细胞受体（TCR）转基因小鼠的完整淋巴结（LN）1的成像研究报告称，T细胞在与相关抗原呈递细胞（APC）接触时会降低其运动性。使用体内双光子成像的T细胞在关节引流（JD）淋巴结，我们检查是否在野生型小鼠中观察到类似的变化T细胞运动。将来自未处理小鼠的T细胞和来自具有蛋白聚糖（PG）诱导的关节炎的小鼠的抗原经历的T细胞共转移到未处理或关节炎受体中，导致关节内抗原（PG）注射后抗原经历的T细胞与APC的相互作用延长，表明来自关节炎野生型小鼠的T细胞重现了在未处理TCR转基因小鼠中报道的运动行为。然而，幼稚T细胞也与关节炎受体的JDLN中的APC进行稳定的相互作用，这表明关节炎宿主的JDLN中的APC将抗原呈递给幼稚或抗原经历的T细胞的能力增强。
Recent imaging studies on intact lymph nodes (LNs)1 of naïve T-cell receptor (TCR)-transgenic mice have reported that T cells reduce their motility upon contact with relevant antigen-presenting cells (APCs). Using in vivo two-photon imaging of T cells in joint-draining (JD) LNs, we examined whether similar changes in T cell motility are observed in wild type mice. Co-transfer of T cells from naïve mice and antigen-experienced T cells from mice with proteoglycan (PG)-induced arthritis into naïve or arthritic recipients resulted in prolonged interactions of antigen-experienced T cells with APCs upon intra-articular antigen (PG) injection, indicating that T cells from arthritic wild type mice recapitulate the motile behavior reported in naïve TCR-transgenic mice. However, naïve T cells also engaged in stable interactions with APCs in the JDLNs of arthritic recipients, suggesting an enhanced ability of APCs in the JDLNs of arthritic hosts to present antigen to either naïve or antigen-experienced T cells.
登录
查看更多内容
影响因子：
--
作者：
Berlo, Suzanne E.;Guichelaar, Teun;Glant, Tibor T.
通讯作者：
Glant, Tibor T.
DOI：
10.1073/pnas.2628040100
发表时间：
2003-03-04
影响因子：
11.1
作者：
Miller, MJ;Wei, SH;Parker, I
通讯作者：
Parker, I
影响因子：
2.2
作者：
Lutz, MB;Kukutsch, N;Schuler, G
通讯作者：
Schuler, G
影响因子：
30.5
作者：
Henrickson, Sarah E.;Mempel, Thorsten R.;von Andrian, Ulrich H.
通讯作者：
von Andrian, Ulrich H.
影响因子：
32.4
作者：
Celli, Susanna;Lemaitre, Fabrice;Bousso, Philippe
通讯作者：
Bousso, Philippe